{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:49:46Z","timestamp":1790434186188,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 2011-331"],"award-info":[{"award-number":["PIP 2011-331"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005746","name":"Universidad Nacional del Litoral","doi-asserted-by":"publisher","award":["CAID"],"award-info":[{"award-number":["CAID"]}],"id":[{"id":"10.13039\/501100005746","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Candida guilliermondii<\/jats:named-content>\n            shows intrinsic reduced echinocandin susceptibility. It harbors two polymorphisms (L633M and T634A) in the Fks1p hot spot 1 region. Our objective was to confirm that the reduced echinocandin susceptibility of\n            <jats:named-content content-type=\"genus-species\">C. guilliermondii<\/jats:named-content>\n            is due to those naturally occurring substitutions. We constructed a\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            mutant in which a region of the\n            <jats:italic>FKS1<\/jats:italic>\n            gene (including hot spot 1) was replaced with that from\n            <jats:named-content content-type=\"genus-species\">C. guilliermondii<\/jats:named-content>\n            . The chimeric mutants showed 32-fold increases in echinocandin MIC values, confirming the hypothesis.\n          <\/jats:p>","DOI":"10.1128\/aac.02644-16","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T01:40:27Z","timestamp":1488246027000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility"],"prefix":"10.1128","volume":"61","author":[{"given":"Catiana","family":"Dudiuk","sequence":"first","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas, Centro Cient\u00edfico Tecnol\u00f3gico Santa Fe, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiana","family":"Macedo","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Leonardelli","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas, Centro Cient\u00edfico Tecnol\u00f3gico Santa Fe, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Theill","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matias S.","family":"Cabeza","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas, Centro Cient\u00edfico Tecnol\u00f3gico Santa Fe, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soledad","family":"Gamarra","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Garcia-Effron","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas, Centro Cient\u00edfico Tecnol\u00f3gico Santa Fe, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12104"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa066906"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-261"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-14-97"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ791"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01901-07"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01590-09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00262-08"},{"key":"e_1_3_2_10_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1998","unstructured":"Sambrook J FritschEFManiatisT. 1998. 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Species distribution and susceptibility profile of yeasts isolated from blood cultures: results of a multicenter active laboratory-based surveillance study in Argentina. 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