{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:54:01Z","timestamp":1762376041866,"version":"3.37.3"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100018789","name":"Child Health Research Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100018789","id-type":"DOI","asserted-by":"crossref"}]},{"name":"John Flatley"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Studies of cystic fibrosis (CF) patient exacerbations attributed to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>\n            infection have indicated a lack of correlation of outcome with\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility results. One explanation is that the media used for testing do not mimic the airway milieu, resulting in incorrect conclusions. Therefore, media have been devised to mimic CF sputum.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus fumigatus<\/jats:named-content>\n            is the leading fungal pathogen in CF, and susceptibility testing is also used to decide therapeutic choices. We assessed whether media designed to mimic CF sputa would give different fungal susceptibility results than those of classical methods, assaying voriconazole, the most utilized anti-\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus<\/jats:named-content>\n            drug in this setting, and 30 CF\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus<\/jats:named-content>\n            isolates. The frequency of marked resistance (defined as an MIC of &gt;4 \u03bcg\/ml) in our CF unit by classical methods is 7%. Studies performed with classical methods and with digested sputum medium, synthetic sputum medium, and artificial sputum medium revealed prominent differences in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus<\/jats:named-content>\n            susceptibility results, as well as growth rate, with each medium. Clinical correlative studies are required to determine which results are most useful in predicting outcome. Comparison of MICs with non-CF isolates also indicated the CF isolates were generally more resistant.\n          <\/jats:p>","DOI":"10.1128\/aac.02649-15","type":"journal-article","created":{"date-parts":[[2016,1,26]],"date-time":"2016-01-26T03:49:13Z","timestamp":1453780153000},"page":"2180-2184","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Effect of Media Modified To Mimic Cystic Fibrosis Sputum on the Susceptibility of Aspergillus fumigatus, and the Frequency of Resistance at One Center"],"prefix":"10.1128","volume":"60","author":[{"given":"David A.","family":"Stevens","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA"},{"name":"California Institute for Medical Research, San Jose, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard B.","family":"Moss","sequence":"additional","affiliation":[{"name":"Division of Pulmonology, Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathy","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Division of Pulmonology, Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl V.","family":"Clemons","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA"},{"name":"California Institute for Medical Research, San Jose, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marife","family":"Martinez","sequence":"additional","affiliation":[{"name":"California Institute for Medical Research, San Jose, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2011-201498"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e328365ab74"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2012.695454"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e328358d50b"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201407-1277OC"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2011.161117"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.1950080106"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.63.8.953"},{"key":"e_1_3_2_10_2","first-page":"105","article-title":"Frequency of Aspergillus fumigatus isolates and antibodies to Aspergillus antigens in cystic fibrosis","volume":"93","author":"Schonheyder H","year":"1985","unstructured":"Schonheyder H, Jensen T, H\u00f8iby N, Andersen P, Koch C. 1985. 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