{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:30:12Z","timestamp":1791639012981,"version":"4.3.4"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Free-living amoebae of the genus\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba<\/jats:named-content>\n            are the causal agents of a sight-threatening ulceration of the cornea called\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba<\/jats:named-content>\n            keratitis, as well as the rare but usually fatal disease granulomatous amoebic encephalitis. Although there are many therapeutic options for the treatment of\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba<\/jats:named-content>\n            infections, they are generally lengthy and\/or have limited efficacy. For the best clinical outcome, treatments should target both the trophozoite and the cyst stages, as cysts are known to confer resistance to treatment. In this study, we document the activities of caffeine and maslinic acid against both the trophozoite and the cyst stages of three clinical strains of\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba<\/jats:named-content>\n            . These drugs were chosen because they are reported to inhibit glycogen phosphorylase, which is required for encystation. Maslinic acid is also reported to be an inhibitor of extracellular proteases, which may be relevant since the protease activities of\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba<\/jats:named-content>\n            species are correlated with their pathogenicity. We also provide evidence for the first time that both drugs exert their anti-amoebal effects through programmed cell death.\n          <\/jats:p>","DOI":"10.1128\/aac.02660-16","type":"journal-article","created":{"date-parts":[[2017,3,21]],"date-time":"2017-03-21T00:35:18Z","timestamp":1490056518000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Amoebicidal Activity of Caffeine and Maslinic Acid by the Induction of Programmed Cell Death in Acanthamoeba"],"prefix":"10.1128","volume":"61","author":[{"given":"Carmen M.","family":"Mart\u00edn-Navarro","sequence":"first","affiliation":[{"name":"University Institute of Tropical Diseases and Public Health of the Canary Islands, Universidad de La Laguna, Tenerife, Canary Islands, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atteneri","family":"L\u00f3pez-Arencibia","sequence":"additional","affiliation":[{"name":"University Institute 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