{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T23:54:31Z","timestamp":1721606071924},"reference-count":39,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            It has been argued that the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of caspofungin (CSP) is not a good predictor of the outcome of echinocandin treatment\n            <jats:italic>in vivo<\/jats:italic>\n            . We evaluated the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of CSP and the presence of\n            <jats:italic>FKS<\/jats:italic>\n            mutations in the hot spot 1 (HS1) region of the\n            <jats:italic>FKS1<\/jats:italic>\n            and\n            <jats:italic>FKS2<\/jats:italic>\n            genes in 17 C\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">andida glabrata<\/jats:named-content>\n            strains with a wide range of MICs. The efficacy of CSP against systemic infections from each of the 17 strains was evaluated in a murine model. No HS1 mutations were found in the eight strains showing MICs for CSP of \u22640.5 \u03bcg\/ml, but they were present in eight of the nine strains with MICs of \u22651 \u03bcg\/ml, i.e., three in the\n            <jats:italic>FKS1<\/jats:italic>\n            gene and five in the\n            <jats:italic>FKS2<\/jats:italic>\n            gene. CSP was effective for treating mice infected with strains with MICs of \u22640.5 \u03bcg\/ml, showed variable efficacy in animals challenged with strains with MICs of 1 \u03bcg\/ml, and did not work in those with strains with MICs of &gt;1 \u03bcg\/ml. In addition, mutations, including one reported for the first time, were found outside the HS1 region in the\n            <jats:italic>FKS2<\/jats:italic>\n            gene of six strains with different MICs, but their presence did not influence drug efficacy. The\n            <jats:italic>in vitro<\/jats:italic>\n            activity of CSP was compared with that of another echinocandin, anidulafungin, suggesting that the MICs of both drugs, as well as mutations in the HS1 regions of the\n            <jats:italic>FKS1<\/jats:italic>\n            and\n            <jats:italic>FKS2<\/jats:italic>\n            genes, are predictive of outcome.\n          <\/jats:p>","DOI":"10.1128\/aac.02666-13","type":"journal-article","created":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T03:30:53Z","timestamp":1397532653000},"page":"3646-3649","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["<i>In Vitro<\/i>\n            Antifungal Susceptibility of Candida glabrata to Caspofungin and the Presence of\n            <i>FKS<\/i>\n            Mutations Correlate with Treatment Response in an Immunocompromised Murine Model of Invasive Infection"],"prefix":"10.1128","volume":"58","author":[{"given":"Fabiola","family":"Fern\u00e1ndez-Silva","sequence":"first","affiliation":[{"name":"Unitat d'Anatomia Patol\u00f2gica, Facultat de Medicina i Ci\u00e8ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Lackner","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Capilla","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Facultat de Medicina i Ci\u00e8ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"Mayayo","sequence":"additional","affiliation":[{"name":"Unitat d'Anatomia Patol\u00f2gica, Facultat de Medicina i Ci\u00e8ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deanna","family":"Sutton","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"Castanheira","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette W.","family":"Fothergill","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Lass-Fl\u00f6rl","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, 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