{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:39:48Z","timestamp":1773283188937,"version":"3.50.1"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The whole sequence of plasmid pENVA carrying the extended-spectrum \u03b2-lactamase gene\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            was determined. It was identified from a series of clonally related\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            sequence type 274 strains recovered from companion animals. This plasmid was 253,984 bp in size and harbored, in addition to\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            , a large array of genes encoding resistance to many antibiotic molecules, including \u03b2-lactams (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-1<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DHA-1<\/jats:sub>\n            ), aminoglycosides (\n            <jats:italic>aacA2<\/jats:italic>\n            ,\n            <jats:italic>aadA1<\/jats:italic>\n            ), tetracycline (\n            <jats:italic>tetA<\/jats:italic>\n            ), quinolones (\n            <jats:italic>qnrB4<\/jats:italic>\n            ), trimethoprim (\n            <jats:italic>dfrA15<\/jats:italic>\n            ), and sulfonamides (two copies of\n            <jats:italic>sul1<\/jats:italic>\n            ). In addition, genes encoding resistance to mercury, tellurium, nickel, and quaternary compounds were identified. It also carried genes encoding DNA damage protection and mutagenesis repair and a locus for a CRISPR system, which corresponds to an immune system involved in protection against bacteriophages and plasmids. Comparative analysis of the plasmid scaffold showed that it possessed a structure similar to that of only a single plasmid, which was pNDM-MAR encoding the carbapenemase NDM-1 and identified from human\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            isolates. Both plasmids possessed two replicons, namely, those of IncFIB-like and IncHIB-like plasmids, which were significantly different from those previously characterized. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            gene, together with the other antibiotic resistance genes, was part of a large module likely acquired through a transposition process. We characterized here a new plasmid type carrying the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            gene identified in a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            isolate of animal origin. The extent to which this plasmid type may spread efficiently and possibly further enhance the dissemination of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            among animal and human isolates remains to be determined.\n          <\/jats:p>","DOI":"10.1128\/aac.02695-14","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T03:52:32Z","timestamp":1398138752000},"page":"3768-3773","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["IncH-Type Plasmid Harboring\n            <i>bla<\/i>\n            <sub>CTX-M-15<\/sub>\n            ,\n            <i>bla<\/i>\n            <sub>DHA-1<\/sub>\n            , and\n            <i>qnrB4<\/i>\n            Genes Recovered from Animal Isolates"],"prefix":"10.1128","volume":"58","author":[{"given":"Andreas","family":"Schl\u00fcter","sequence":"first","affiliation":[{"name":"Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"INSERM U914, South-Paris Medical School, Le Kremlin-Bic\u00eatre, France"},{"name":"Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9my A.","family":"Bonnin","sequence":"additional","affiliation":[{"name":"INSERM U914, South-Paris Medical School, Le Kremlin-Bic\u00eatre, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves","family":"Millemann","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Microbiologie Alimentaire, S\u00e9curit\u00e9 et Qualit\u00e9 des Aliments (MASQ), Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, Universit\u00e9 Paris-Est, Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix G.","family":"Eikmeyer","sequence":"additional","affiliation":[{"name":"Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Wibberg","sequence":"additional","affiliation":[{"name":"Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfred","family":"P\u00fchler","sequence":"additional","affiliation":[{"name":"Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"INSERM U914, South-Paris 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