{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:57:51Z","timestamp":1792126671548,"version":"4.4.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Klebsiella pneumoniae<\/jats:named-content>\n carbapenemase (KPC)-producing\n K. pneumoniae<\/jats:named-content>\n strains have spread worldwide and become a major threat in health care facilities. Transmission of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n , the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide sequences of two novel\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n -harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length, with a mosaic plasmid structure; it exhibits homologies to several other plasmids but lacks the plasmid transfer operon (\n tra<\/jats:italic>\n ) and the origin of transfer (\n oriT<\/jats:italic>\n ) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrated plasmid structure, comprising a 72-kb element that highly resembles pBK30661 (>99.9% nucleotide identities) and an extra 68-kb element that harbors\n tra<\/jats:italic>\n and\n oriT<\/jats:italic>\n . A PCR scheme was designed to detect the distribution of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n -harboring IncFIA (pBK30661-like and pBK30683-like) plasmids in a collection of clinical\n Enterobacteriaceae<\/jats:named-content>\n isolates from 10 hospitals in New Jersey and New York. KPC-harboring IncFIA plasmids were found in 20% of 491\n K. pneumoniae<\/jats:named-content>\n isolates, and all carried\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n . pBK30661-like plasmids were identified mainly in the epidemic sequence type 258 (ST258)\n K. pneumoniae<\/jats:named-content>\n clone, while pBK30683-like plasmids were widely distributed in ST258 and other\n K. pneumoniae<\/jats:named-content>\n sequence types and among non-\n K. pneumoniae Enterobacteriaceae<\/jats:named-content>\n species. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n -harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and to decipher the origins of pBK30661-like and pBK30683-like plasmids.\n <\/jats:p>","DOI":"10.1128\/aac.02749-13","type":"journal-article","created":{"date-parts":[[2014,2,3]],"date-time":"2014-02-03T23:05:07Z","timestamp":1391468707000},"page":"2289-2294","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Molecular Survey of the Dissemination of Two\n bla<\/i>\n KPC<\/sub>\n -Harboring IncFIA Plasmids in New Jersey and New York Hospitals"],"prefix":"10.1128","volume":"58","author":[{"given":"Liang","family":"Chen","sequence":"first","affiliation":[{"name":"Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyan D.","family":"Chavda","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto G.","family":"Melano","sequence":"additional","affiliation":[{"name":"Ontario Agency for Health Protection and Promotion, Toronto, Ontario, Canada"},{"name":"University of Toronto, Toronto, Ontario, Canada"},{"name":"Mount Sinai Hospital, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hackensack Medical Center, Hackensack, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert D.","family":"Rojtman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Jersey Shore University Medical Center, Neptune, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA"},{"name":"Departments of Medicine, Pharmacology, Molecular Biology, and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry N.","family":"Kreiswirth","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70190-7"},{"key":"e_1_3_2_3_2","first-page":"165","article-title":"Vital signs: carbapenem-resistant Enterobacteriaceae","volume":"62","author":"Jacob JT","year":"2013","unstructured":"JacobJTKleinELaxminarayanRBeldavsZLynfieldRKallenAJRicksPEdwardsJSrinivasanAFridkinSRasheedKJLonswayDBulensSHerreraRMcDonaldLCPatelJLimbagoBBellMCardoD. 2013. Vital signs: carbapenem-resistant Enterobacteriaceae. MMWR Morb. Mortal. Wkly. Rep. 62:165\u2013170.","journal-title":"MMWR Morb. Mortal. Wkly. 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