{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:18:12Z","timestamp":1791461892139,"version":"4.3.4"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Titanium (Ti) is a promising candidate biomaterial for an artificial corneal skirt. Antimicrobial peptide (AMP) immobilization may improve the bactericidal effect of the Ti substrate. In this study, we tested the bactericidal efficacy of a functionalized Ti surface in a rabbit keratitis model. A corneal stromal pocket was created by a femtosecond laser. The Ti films were then inserted into the pocket, and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            or\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>\n            was inoculated into the pocket above the implant films. The corneas with Ti-AMP implants were compared with the corneas implanted with unprotected Ti by slit lamp observation and anterior segment optical coherence tomography (AS-OCT). Inflammatory responses were evaluated by bacterium counting, hematoxylin-eosin staining, and immunostaining. There was a lower incidence and a lesser extent of infection on rabbit corneas with Ti-AMP implants than on those with unprotected Ti implants. The bactericidal effect of AMP against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n            was comparable to that of postoperative prophylactic antibiotic treatment; hence, SESB2V AMP bound to the Ti implant provided functional activity\n            <jats:italic>in vivo<\/jats:italic>\n            , but its efficacy was greater against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n            than against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>\n            . This work suggests that SESB2V AMP can be successfully functionalized in a rabbit keratitis model to prevent perioperative corneal infection.\n          <\/jats:p>","DOI":"10.1128\/aac.02859-14","type":"journal-article","created":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T06:08:33Z","timestamp":1403590113000},"page":"5229-5238","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Effectiveness of Antimicrobial Peptide Immobilization for Preventing Perioperative Cornea Implant-Associated Bacterial Infection"],"prefix":"10.1128","volume":"58","author":[{"given":"Xiao Wei","family":"Tan","sequence":"first","affiliation":[{"name":"Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tze Wei","family":"Goh","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Saraswathi","sequence":"additional","affiliation":[{"name":"Antimicrobials Group, Singapore Eye Research Institute, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan Lwin","family":"Nyein","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melina","family":"Setiawan","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andri","family":"Riau","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Lakshminarayanan","sequence":"additional","affiliation":[{"name":"Antimicrobials Group, Singapore Eye Research Institute, Singapore"},{"name":"Duke-NUS Graduate Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouping","family":"Liu","sequence":"additional","affiliation":[{"name":"Antimicrobials Group, Singapore Eye Research Institute, Singapore"},{"name":"Duke-NUS Graduate Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"Tan","sequence":"additional","affiliation":[{"name":"Yong Loo Lin School of Medicine, National University of Singapore, Singapore"},{"name":"Singapore National Eye Centre, Singapore"},{"name":"Duke-NUS Graduate Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger W.","family":"Beuerman","sequence":"additional","affiliation":[{"name":"Antimicrobials Group, Singapore Eye Research Institute, Singapore"},{"name":"Yong Loo Lin School of Medicine, National University of Singapore, Singapore"},{"name":"Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jodhbir S.","family":"Mehta","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore"},{"name":"Antimicrobials Group, Singapore Eye Research Institute, Singapore"},{"name":"Singapore National Eye Centre, Singapore"},{"name":"Duke-NUS Graduate Medical School, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra035415"},{"key":"e_1_3_3_3_2","first-page":"75","volume-title":"Infections associated with indwelling medical devices","author":"Baker AS","year":"1989","unstructured":"BakerASScheinOD. 1989. 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