{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:22:26Z","timestamp":1790198546974,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Antibiotic-resistant<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter<\/jats:named-content>constitutes a serious threat to public health, and resistance to macrolides is of particular concern, as this class of antibiotics is the drug of choice for clinical therapy of campylobacteriosis. Very recently, a horizontally transferrable macrolide resistance mediated by the rRNA methylase gene<jats:italic>erm<\/jats:italic>(B) was reported in a<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter coli<\/jats:named-content>isolate, but little is known about the dissemination of<jats:italic>erm<\/jats:italic>(B) among<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter<\/jats:named-content>isolates and the association of<jats:italic>erm<\/jats:italic>(B)-carrying isolates with clinical disease. To address this question and facilitate the control of antibiotic-resistant<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter<\/jats:named-content>, we determined the distribution of<jats:italic>erm<\/jats:italic>(B) in 1,554<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. coli<\/jats:named-content>and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter jejuni<\/jats:named-content>isolates derived from food-producing animals and clinically confirmed human diarrheal cases. The results revealed that 58 of the examined isolates harbored<jats:italic>erm<\/jats:italic>(B) and exhibited high-level resistance to macrolides, and most were recent isolates, derived in 2011-2012. In addition, the<jats:italic>erm<\/jats:italic>(B)-positive isolates were all resistant to fluoroquinolones, another clinically important antibiotic used for treating campylobacteriosis. The<jats:italic>erm<\/jats:italic>(B) gene is found to be associated with chromosomal multidrug resistance genomic islands (MDRGIs) of Gram-positive origin or with plasmids of various sizes. All MDRGIs were transferrable to macrolide-susceptible<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>by natural transformation under laboratory conditions. Molecular typing of the<jats:italic>erm<\/jats:italic>(B)-carrying isolates by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) identified diverse genotypes and outbreak-associated diarrheal isolates. Molecular typing also suggested zoonotic transmission of<jats:italic>erm<\/jats:italic>(B)-positive<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter<\/jats:named-content>. These findings reveal an emerging and alarming trend of dissemination of<jats:italic>erm<\/jats:italic>(B) and MDRGIs in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter<\/jats:named-content>and underscore the need for heightened efforts to control their further spread.<\/jats:p>","DOI":"10.1128\/aac.03039-14","type":"journal-article","created":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T14:43:52Z","timestamp":1404225832000},"page":"5405-5412","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Emergence of Multidrug-Resistant Campylobacter Species Isolates with a Horizontally Acquired rRNA Methylase"],"prefix":"10.1128","volume":"58","author":[{"given":"Yang","family":"Wang","sequence":"first","affiliation":[{"name":"National Center for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maojun","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengru","family":"Deng","sequence":"additional","affiliation":[{"name":"National Center for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangqi","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congming","family":"Wu","sequence":"additional","affiliation":[{"name":"National Center for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Shen","sequence":"additional","affiliation":[{"name":"National Center for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/509936"},{"key":"e_1_3_3_3_2","first-page":"99","volume-title":"Campylobacter","author":"Blaser MJ","year":"2008","unstructured":"BlaserMJEngbergJ. 2008. 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