{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T00:57:18Z","timestamp":1751677038045},"reference-count":35,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Strains of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Neisseria gonorrhoeae<\/jats:named-content>\n            with mosaic\n            <jats:italic>penA<\/jats:italic>\n            genes bearing novel point mutations in\n            <jats:italic>penA<\/jats:italic>\n            have been isolated from ceftriaxone treatment failures. Such isolates exhibit significantly higher MIC values to third-generation cephalosporins. Here we report the\n            <jats:italic>in vitro<\/jats:italic>\n            isolation of two mutants with elevated MICs to cephalosporins. The first possesses a point mutation in the transpeptidase region of the mosaic\n            <jats:italic>penA<\/jats:italic>\n            gene, and the second contains an insertion mutation in\n            <jats:italic>pilQ<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.03082-14","type":"journal-article","created":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T03:22:56Z","timestamp":1410232976000},"page":"6986-6989","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["<i>In Vitro<\/i>\n            Selection of Neisseria gonorrhoeae Mutants with Elevated MIC Values and Increased Resistance to Cephalosporins"],"prefix":"10.1128","volume":"58","author":[{"given":"Steven R.","family":"Johnson","sequence":"first","affiliation":[{"name":"Division of STD Prevention, NCHHSTP, Centers for Disease Control and Prevention, Atlanta, Georgia, 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