{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T12:43:24Z","timestamp":1724330604368},"reference-count":17,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n We previously reported the development of a prototype antibiotic sensitivity assay to detect drug-resistant\n Mycobacterium tuberculosis<\/jats:named-content>\n using infection by mycobacteriophage to create a novel nucleic acid transcript, a surrogate marker of mycobacterial viability, detected by reverse transcriptase PCR (M. C. Mulvey et al., mBio\n 3:<\/jats:bold>\n e00312-11, 2012). This assay detects antibiotic resistance to all drugs, even drugs for which the resistance mechanism is unknown or complex: it is a phenotypic readout using nucleic acid detection. In this report, we describe development and characteristics of an optimized reporter system that directed expression of the RNA cyclase ribozyme, which generated circular RNA through an intramolecular splicing reaction and led to accumulation of a new nucleic acid sequence in phage-infected bacteria. These modifications simplified the assay, increased the limit of detection from 10\n 4<\/jats:sup>\n to <10\n 2<\/jats:sup>\n M. tuberculosis<\/jats:named-content>\n cells, and correctly identified the susceptibility profile of\n M. tuberculosis<\/jats:named-content>\n strains exposed for 16 h to either first-line or second-line antitubercular drugs. In addition to phenotypic drug resistance or susceptibility, the assay reported streptomycin MICs and clearly detected 10% drug-resistant cells in an otherwise drug-susceptible population.\n <\/jats:p>","DOI":"10.1128\/aac.03135-14","type":"journal-article","created":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T04:50:09Z","timestamp":1415076609000},"page":"407-413","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Optimization of a Nucleic Acid-Based Reporter System To Detect Mycobacterium tuberculosis Antibiotic Sensitivity"],"prefix":"10.1128","volume":"59","author":[{"given":"Matthew C.","family":"Mulvey","sequence":"first","affiliation":[{"name":"Sequella, Inc., Rockville, Maryland, USA"},{"name":"BeneVir Biopharm, Inc., Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Lemmon","sequence":"additional","affiliation":[{"name":"Sequella, Inc., Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Rotter","sequence":"additional","affiliation":[{"name":"Sequella, Inc., Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Lees","sequence":"additional","affiliation":[{"name":"Sequella, Inc., Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo","family":"Einck","sequence":"additional","affiliation":[{"name":"Sequella, Inc., Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol A.","family":"Nacy","sequence":"additional","affiliation":[{"name":"Sequella, Inc., Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"301","article-title":"Emergence of Mycobacterium tuberculosis with extensive resistance to second-line drugs worldwide, 2000\u20132004","volume":"55","author":"Centers for Disease Control and Prevention","year":"2006","unstructured":"Centers for Disease Control and Prevention. 2006. Emergence of Mycobacterium tuberculosis with extensive resistance to second-line drugs worldwide, 2000\u20132004. MMWR Morb Mortal Wkly Rep 55:301\u2013305.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_2_3_2","volume-title":"Antituberculosis drug resistance in the world: report no. 2, prevalence and trends","author":"WHO\/IUATLD","year":"2000","unstructured":"WHO\/IUATLD. 2000. Antituberculosis drug resistance in the world: report no. 2, prevalence and trends. WHO\/CDS\/TB\/2000.278. 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American Society for Microbiology, Washington, DC."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.3883-3888.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01315-11"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00128-10"},{"key":"e_1_3_2_14_2","first-page":"565","article-title":"Mycobacteria: laboratory methods for testing drug sensitivity and resistance","volume":"29","author":"Canetti G","year":"1963","unstructured":"Canetti G, Froman S, Grosset J, Hauduroy P, Langerova M, Mahler HT, Meissner G, Mitchison DA, Sula L. 1963. Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull World Health Organ 29:565\u2013578.","journal-title":"Bull World Health Organ"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(89)90016-8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.2.290-295.1999"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1119540109"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1208813"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.03135-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.03135-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:53:22Z","timestamp":1645484002000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.03135-14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1128\/AAC.03135-14"],"URL":"https:\/\/doi.org\/10.1128\/aac.03135-14","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1]]},"assertion":[{"value":"2014-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-10-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}