{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:43:09Z","timestamp":1768286589308,"version":"3.49.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Chlamydia trachomatis<\/jats:named-content>\n            is a medically important human pathogen causing different diseases, including trachoma, the leading cause of preventable blindness in developing countries, and sexually transmitted infections that can lead to infertility and ectopic pregnancies. There is no vaccine against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. trachomatis<\/jats:named-content>\n            at present. Broad-spectrum antibiotics are used as standard therapy to treat the infection but have unwanted side effects, such as inducing persistent or recurring infections and affecting the host microbiome, necessitating the development of novel anti-\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Chlamydia<\/jats:named-content>\n            therapies. Here, we describe the establishment of a robust, fast, and simple plaque assay using liquid overlay medium (LOM) for the identification of anti-\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Chlamydia<\/jats:named-content>\n            compounds. Using the LOM plaque assay, we identified nitrobenzoxadiazole (NBD)-labeled 1-\n            <jats:italic>O<\/jats:italic>\n            -methyl-ceramide-C\n            <jats:sub>16<\/jats:sub>\n            as a compound that efficiently inhibits\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. trachomatis<\/jats:named-content>\n            replication without affecting the viability of the host cell. Further detailed analyses indicate that 1-\n            <jats:italic>O<\/jats:italic>\n            -methyl-NBD-ceramide-C\n            <jats:sub>16<\/jats:sub>\n            acts outside the inclusion. Thereby, 1-\n            <jats:italic>O<\/jats:italic>\n            -methyl-NBD-ceramide-C\n            <jats:sub>16<\/jats:sub>\n            represents a lead compound for the development of novel anti-\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Chlamydia<\/jats:named-content>\n            drugs and furthermore constitutes an agent to illuminate sphingolipid trafficking pathways in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Chlamydia<\/jats:named-content>\n            infections.\n          <\/jats:p>","DOI":"10.1128\/aac.03457-14","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T04:16:33Z","timestamp":1404792993000},"page":"5537-5546","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Improved Plaque Assay Identifies a Novel Anti-Chlamydia Ceramide Derivative with Altered Intracellular Localization"],"prefix":"10.1128","volume":"58","author":[{"given":"Sebastian","family":"Banhart","sequence":"first","affiliation":[{"name":"Junior Research Group \u201cSexually Transmitted Bacterial Pathogens\u201d (NG 5), Robert Koch Institute, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Essa M.","family":"Saied","sequence":"additional","affiliation":[{"name":"Organic and Bioorganic Chemistry, Department of Chemistry, Humboldt-Universit\u00e4t zu Berlin, Berlin, Germany"},{"name":"Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Martini","sequence":"additional","affiliation":[{"name":"Junior Research Group \u201cSexually Transmitted Bacterial Pathogens\u201d (NG 5), Robert Koch Institute, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"Koch","sequence":"additional","affiliation":[{"name":"Junior Research Group \u201cSexually Transmitted Bacterial Pathogens\u201d (NG 5), Robert Koch Institute, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Aeberhard","sequence":"additional","affiliation":[{"name":"Junior Research Group \u201cSexually Transmitted Bacterial Pathogens\u201d (NG 5), Robert Koch Institute, Berlin, Germany"},{"name":"Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazimierz","family":"Madela","sequence":"additional","affiliation":[{"name":"Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Arenz","sequence":"additional","affiliation":[{"name":"Organic and Bioorganic Chemistry, Department of Chemistry, Humboldt-Universit\u00e4t zu Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dagmar","family":"Heuer","sequence":"additional","affiliation":[{"name":"Junior Research Group \u201cSexually Transmitted Bacterial Pathogens\u201d (NG 5), Robert Koch Institute, Berlin, 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