{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:06:31Z","timestamp":1791234391576,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n The linkage between the macrolide efflux gene\n mef<\/jats:italic>\n (I) and the chloramphenicol inactivation gene\n catQ<\/jats:italic>\n was first described in\n Streptococcus pneumoniae<\/jats:named-content>\n (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in\n Streptococcus pyogenes<\/jats:named-content>\n (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn\n 5253<\/jats:italic>\n family integrative and conjugative elements (ICEs). The ICE from\n S. pneumoniae<\/jats:named-content>\n (ICE\n Spn<\/jats:italic>\n 529IQ) was sequenced, whereas the ICEs from\n S. pyogenes<\/jats:named-content>\n (ICE\n Spy<\/jats:italic>\n 029IQ and ICE\n Spy<\/jats:italic>\n 005IQ, the first Tn\n 5253<\/jats:italic>\n -like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICE\n Spn<\/jats:italic>\n 529IQ and ICE\n Spy<\/jats:italic>\n 029IQ were found to share the\n \n int\n Sp<\/jats:sub>\n <\/jats:italic>\n 23FST81<\/jats:sub>\n integrase gene and an identical Tn\n 916<\/jats:italic>\n fragment, whereas ICE\n Spy<\/jats:italic>\n 005IQ has\n \n int\n 5252<\/jats:sub>\n <\/jats:italic>\n and lacks Tn\n 916<\/jats:italic>\n . All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn\n 5253<\/jats:italic>\n -like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICE\n Spn<\/jats:italic>\n 529IQ and ICE\n Spy<\/jats:italic>\n 029IQ, and another in ICE\n Spy<\/jats:italic>\n 005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (\n \n int\n Sp<\/jats:sub>\n <\/jats:italic>\n 23FST81<\/jats:sub>\n or\n \n int\n 5252<\/jats:sub>\n <\/jats:italic>\n ). Only ICE\n Spy<\/jats:italic>\n 005IQ was excised in circular form and transferred by conjugation. By transformation,\n mef<\/jats:italic>\n (I) and\n catQ<\/jats:italic>\n were cotransferred at a high frequency from\n S. pyogenes<\/jats:named-content>\n Spy005 and at very low frequencies from\n S. pneumoniae<\/jats:named-content>\n Spn529 and\n S. pyogenes<\/jats:named-content>\n Spy029.\n <\/jats:p>","DOI":"10.1128\/aac.03638-14","type":"journal-article","created":{"date-parts":[[2014,7,29]],"date-time":"2014-07-29T06:34:28Z","timestamp":1406615668000},"page":"5886-5893","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Tn\n 5253<\/i>\n Family Integrative and Conjugative Elements Carrying\n mef<\/i>\n (I) and\n catQ<\/i>\n Determinants in Streptococcus pneumoniae and Streptococcus pyogenes"],"prefix":"10.1128","volume":"58","author":[{"given":"Marina","family":"Mingoia","sequence":"first","affiliation":[{"name":"Unit of Microbiology, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche Medical School, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Morici","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche Medical School, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Morroni","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche Medical School, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Giovanetti","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Del Grosso","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Pantosti","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro E.","family":"Varaldo","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche Medical School, Ancona, 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