{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:53:16Z","timestamp":1792068796776,"version":"4.4.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The POM-1 metallo-\u03b2-lactamase is a subclass B3 resident enzyme produced by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas otitidis<\/jats:named-content>\n                    , a pathogen causing otic infections. The enzyme was overproduced in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n                    BL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.\n                  <\/jats:p>","DOI":"10.1128\/aac.03843-14","type":"journal-article","created":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T22:19:23Z","timestamp":1418681963000},"page":"1755-1758","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Biochemical Characterization of the POM-1 Metallo-\u03b2-Lactamase from Pseudomonas otitidis"],"prefix":"10.1128","volume":"59","author":[{"given":"Luisa","family":"Borgianni","sequence":"first","affiliation":[{"name":"Dipartimento di Biotecnologie Mediche, Universit\u00e0 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"De Luca","sequence":"additional","affiliation":[{"name":"Dipartimento di Biotecnologie Mediche, Universit\u00e0 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Thaller","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia, Universit\u00e0 di Roma Tor Vergata, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunsop","family":"Chong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biotecnologie Mediche, Universit\u00e0 di Siena, Siena, Italy"},{"name":"Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy"},{"name":"Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Denis","family":"Docquier","sequence":"additional","affiliation":[{"name":"Dipartimento di Biotecnologie Mediche, Universit\u00e0 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(11)70056-1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01062-10"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63753-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.11.007"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/201389"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01336-09"},{"key":"e_1_3_2_8_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. 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