{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:34:18Z","timestamp":1791261258144,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycoplasma bovis<\/jats:named-content>\n                    isolates with decreased susceptibilities to tetracyclines are increasingly reported worldwide. The acquired molecular mechanisms associated with this phenomenon were investigated in 70 clinical isolates of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. bovis<\/jats:named-content>\n                    . Sequence analysis of the two 16S rRNA-encoding genes (\n                    <jats:italic>rrs3<\/jats:italic>\n                    and\n                    <jats:italic>rrs4<\/jats:italic>\n                    alleles) containing the primary binding pocket for tetracycline (Tet-1 site) was performed on isolates with tetracycline hydrochloride MICs of 0.125 to 16 \u03bcg\/ml. Mutations at positions A965T, A967T\/C (\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n                    numbering) of helix 31, U1199C of helix 34, and G1058A\/C were identified. Decreased susceptibilities to tetracycline (MICs, \u22652 \u03bcg\/ml) were associated with mutations present at two (A965 and A967) or three positions (A965, A967, and G1058) of the two\n                    <jats:italic>rrs<\/jats:italic>\n                    alleles. No\n                    <jats:italic>tet<\/jats:italic>\n                    (M),\n                    <jats:italic>tet<\/jats:italic>\n                    (O), or\n                    <jats:italic>tet<\/jats:italic>\n                    (L) determinants were found in the genome of any of the 70\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. bovis<\/jats:named-content>\n                    isolates. The data presented correlate (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.0001) the mutations identified in the Tet-1 site of clinical isolates of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. bovis<\/jats:named-content>\n                    with decreased susceptibility to tetracycline.\n                  <\/jats:p>","DOI":"10.1128\/aac.03876-14","type":"journal-article","created":{"date-parts":[[2014,11,18]],"date-time":"2014-11-18T00:07:10Z","timestamp":1416269230000},"page":"796-802","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["16S rRNA Gene Mutations Associated with Decreased Susceptibility to Tetracycline in Mycoplasma bovis"],"prefix":"10.1128","volume":"59","author":[{"given":"E.","family":"Amram","sequence":"first","affiliation":[{"name":"Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel"},{"name":"Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Mikula","sequence":"additional","affiliation":[{"name":"Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Schnee","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institute, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. D.","family":"Ayling","sequence":"additional","affiliation":[{"name":"Animal and Plant Health Agency, Addlestone, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A. J.","family":"Nicholas","sequence":"additional","affiliation":[{"name":"Animal and Plant Health Agency, Addlestone, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. S.","family":"Rosales","sequence":"additional","affiliation":[{"name":"Animal and Plant Health Agency, Addlestone, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Harrus","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Lysnyansky","sequence":"additional","affiliation":[{"name":"Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(02)00155-8"},{"key":"e_1_3_2_3_2","first-page":"231","volume-title":"Antimicrobial therapy in veterinary medicine","author":"Giguere S","year":"2007","unstructured":"Giguere S. 2007. Tetracyclines and glycylcyclines, p 231\u2013240. In Giguere S, Prescott JF, Baggot JD, Walker RD, Dowling PM (ed), Antimicrobial therapy in veterinary medicine, 4th ed. Wiley Blackwell, Hoboken, NJ.","edition":"4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","DOI":"10.1128\/9781555815929","volume-title":"Antimicrobial agents: antibacterials and antifungals","author":"Brysker A","year":"2005","unstructured":"Brysker A (ed). 2005. Antimicrobial agents: antibacterials and antifungals. 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