{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:23:59Z","timestamp":1791307439354,"version":"4.3.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated doripenem-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:named-content>\n            -\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter calcoaceticus<\/jats:named-content>\n            complex (ACB;\n            <jats:italic>n<\/jats:italic>\n            = 411) and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 92) isolates collected from patients from 14 European and Mediterranean countries during 2009 to 2011 for the presence of carbapenemase-encoding genes and clonality. Following susceptibility testing, carbapenem-resistant (doripenem MIC, &gt;2 \u03bcg\/ml) isolates were screened for carbapenemases. New \u03b2-lactamase genes were expressed in a common background and susceptibility was tested. Class 1 integrons were sequenced. Clonality was evaluated by pulsed-field gel electrophoresis and multilocus sequence typing (Pasteur scheme). Relative expression of \u03b2-lactam intrinsic resistance mechanisms was determined for carbapenemase-negative\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            . ACB and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            displayed 58.9 and 0.9% doripenem resistance, respectively.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-24\/OXA-40<\/jats:sub>\n            were detected among 277, 77, and 29 ACB, respectively (in 8, 6, and 5 countries). Ten Turkish isolates carried\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-11<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-22<\/jats:sub>\n            . GES-22 (G243A and M169L mutations in GES-1) had an extended-spectrum \u03b2-lactamase profile. A total of 33 clusters of \u22652 ACB isolates were observed, and 227 isolates belonged to sequence type 2\/international clone II. Other international clones were limited to Turkey and Israel. Doripenem-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            increased significantly (0.7 to 1.6%), and 15\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            - and 22\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-3<\/jats:sub>\n            -carrying isolates, mostly belonging to clonal complexes 11 and 258, were observed.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            isolates producing OXA-48 (\n            <jats:italic>n<\/jats:italic>\n            = 16; in Turkey and Italy), VIM-1 (\n            <jats:italic>n<\/jats:italic>\n            = 10; in Greece, Poland, and Spain), VIM-26 (\n            <jats:italic>n<\/jats:italic>\n            = 1; in Greece), and IMP-19, VIM-4, and the novel VIM-35 (\n            <jats:italic>n<\/jats:italic>\n            = 1 each from Poland) were detected. VIM-35 had one substitution compared to VIM-1 (A235T) and a similar susceptibility profile. One or more resistance mechanisms were identified in 4\/6 carbapenemase-negative\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            . This broad evaluation confirms results from country-specific surveys and shows a highly diverse population of carbapenemase-producing ACB and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            in Europe and Mediterranean countries.\n          <\/jats:p>","DOI":"10.1128\/aac.03930-14","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T02:09:54Z","timestamp":1412042994000},"page":"7358-7366","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Evaluation of Clonality and Carbapenem Resistance Mechanisms among Acinetobacter baumannii-Acinetobacter calcoaceticus Complex and Enterobacteriaceae Isolates Collected in European and Mediterranean Countries and Detection of Two Novel \u03b2-Lactamases, GES-22 and VIM-35"],"prefix":"10.1128","volume":"58","author":[{"given":"Mariana","family":"Castanheira","sequence":"first","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Costello","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah N.","family":"Woosley","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalitagauri M.","family":"Deshpande","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd A.","family":"Davies","sequence":"additional","affiliation":[{"name":"Janssen Research &amp; Development, Raritan, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald N.","family":"Jones","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks063"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2165\/11315690-000000000-00000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/595011"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2012.06.001"},{"key":"e_1_3_2_6_2","volume-title":"Antimicrobial resistance surveillance in Europe 2012","author":"EARS-Net","year":"2012","unstructured":"EARS-Net. 2012. 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