{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:07:44Z","timestamp":1790694464108,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Extrapulmonary and, in particular, spinal tuberculosis (TB) constitutes a minor but significant part of the total TB incidence. In spite of this, almost no studies on the genetic diversity and drug resistance of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>\n            isolates from spinal TB patients have been published to date. Here, we report results of the first Russian and globally largest molecular study of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. tuberculosis<\/jats:named-content>\n            isolates recovered from patients with tuberculous spondylitis (TBS). The majority of 107 isolates were assigned to the Beijing genotype (\n            <jats:italic>n<\/jats:italic>\n            = 80); the other main families were T (\n            <jats:italic>n<\/jats:italic>\n            = 11), Ural (\n            <jats:italic>n<\/jats:italic>\n            = 7), and LAM (\n            <jats:italic>n<\/jats:italic>\n            = 4). Multidrug resistance (MDR) was more frequently found among Beijing (90.5%) and, intriguingly, Ural (71.4%) isolates than other genotypes (5%;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). The extremely drug-resistant (XDR) phenotype was exclusively found in the Beijing isolates (\n            <jats:italic>n<\/jats:italic>\n            = 7). A notable prevalence of the\n            <jats:italic>rpoB531<\/jats:italic>\n            and\n            <jats:italic>katG315<\/jats:italic>\n            mutations in Beijing strains that were similarly high in both TBS (this study) and published pulmonary TB (PTB) samples from Russia shows that TBS and PTB Beijing strains follow the same paradigm of acquisition of rifampin (RIF) and isoniazid (INH) resistance. The 24-locus mycobacterial interspersed repetitive unit\u2013variable-number tandem-repeat (MIRU-VNTR) subtyping of 80 Beijing isolates further discriminated them into 24 types (Hunter Gaston index [HGI] = 0.83); types 100-32 and 94-32 represented the largest groups. A genotype of Russian successful clone B0\/W148 was identified in 30 of 80 Beijing isolates. In conclusion, this study highlighted a crucial impact of the Beijing genotype and the especially prominent role of its MDR-associated successful clone B0\/W148 cluster in the development of spinal MDR-TB in Russian patients.\n          <\/jats:p>","DOI":"10.1128\/aac.04221-14","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T06:04:54Z","timestamp":1422943494000},"page":"2349-2357","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Tuberculous Spondylitis in Russia and Prominent Role of Multidrug-Resistant Clone Mycobacterium tuberculosis Beijing B0\/W148"],"prefix":"10.1128","volume":"59","author":[{"given":"Anna","family":"Vyazovaya","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Mokrousov","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Solovieva","sequence":"additional","affiliation":[{"name":"Research Institute of Phthisiopulmonology, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Mushkin","sequence":"additional","affiliation":[{"name":"Research Institute of Phthisiopulmonology, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Manicheva","sequence":"additional","affiliation":[{"name":"Research Institute of Phthisiopulmonology, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Vishnevsky","sequence":"additional","affiliation":[{"name":"Research Institute of Phthisiopulmonology, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viacheslav","family":"Zhuravlev","sequence":"additional","affiliation":[{"name":"Research Institute of Phthisiopulmonology, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Narvskaya","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia"},{"name":"Research Institute of Phthisiopulmonology, St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"WHO report","author":"World Health Organization (WHO)","year":"2012","unstructured":"World Health Organization (WHO). 2012. Global tuberculosis control: WHO report. WHO, Geneva, Switzerland."},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","unstructured":"Solovic I Jonsson J Korzeniewska-Kose\u0142a M Chiotan DI Pace-Asciak A Slump E Rumetshofer R Abubakar I Kos S Svetina-Sorli P Haas W Bauer T Sandgren A van der Werf MJ. 2013. Challenges in diagnosing extrapulmonary tuberculosis in the European Union 2011. Euro Surveill 18:pii=20432. http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId=20432.","DOI":"10.2807\/ese.18.12.20432-en"},{"key":"e_1_3_3_4_2","volume-title":"Information Proceedings of Statistical and Analytical Data, part 3","year":"2012","unstructured":". 2012. Infectious morbidity in the subjects of Russian Federation in 2010\u20132011. In Information Proceedings of Statistical and Analytical Data, part 3. Federal Center of Hygiene and Epidemiology, Moscow, Russia. (In Russian.)"},{"key":"e_1_3_3_5_2","first-page":"3","article-title":"Extrapulmonary tuberculosis in Russia: official statistics and reality","volume":"2006","author":"Levashev YN","year":"2006","unstructured":"Levashev YN, Mushkin AY, Grishko AN. 2006. Extrapulmonary tuberculosis in Russia: official statistics and reality. Probl Tuberk Bolezn Legk 2006:3\u20136. (In Russian.)","journal-title":"Probl Tuberk Bolezn Legk"},{"key":"e_1_3_3_6_2","first-page":"17","article-title":"Tuberculosis of extrapulmonary localization based on personal registers of newly diagnosed patients","volume":"2009","author":"Kartavykh AA","year":"2009","unstructured":"Kartavykh AA, Borisov SE, Matveeva MV. 2009. Tuberculosis of extrapulmonary localization based on personal registers of newly diagnosed patients. Tuberk Bolezn Legk 2009:17\u201326. (In Russian.)","journal-title":"Tuberk Bolezn Legk"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1179\/2045772311Y.0000000023"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4184\/asj.2012.6.4.294"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2011.12.023"},{"key":"e_1_3_3_10_2","first-page":"39","article-title":"Genetic marking of polyresistant Mycobacterium tuberculosis strains isolated in the North-West of Russia","volume":"1999","author":"Narvskaia O","year":"1999","unstructured":"Narvskaia O, Mokrousov I, Otten TF, Vyshnevskiy BI. 1999. Genetic marking of polyresistant Mycobacterium tuberculosis strains isolated in the North-West of Russia. Probl Tuberk 1999:39\u201341. (In Russian.)","journal-title":"Probl Tuberk"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00087-12"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2012.10.005"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.2.406-409.1993"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.4.907-914.1997"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01392-06"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.02.004"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02231-07"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01485-13"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2008.07.003"},{"key":"e_1_3_3_20_2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1017\/CBO9781139003308.019","volume-title":"Causes and consequences of human migration","author":"Mokrousov I","year":"2012","unstructured":"Mokrousov I. 2012. Human migratory history: through the looking-glass of genetic geography of Mycobacterium tuberculosis, p 317\u2013341. In Crawford MH, Campbell B (ed), Causes and consequences of human migration. Cambridge University Press, Cambridge, United Kingdom."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02001-12"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01183.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00439-06"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-90"},{"key":"e_1_3_3_25_2","first-page":"430","article-title":"Extensively drug-resistant tuberculosis (XDR.TB): recommendations for prevention and control","volume":"81","author":"World Health Organization (WHO)","year":"2006","unstructured":"World Health Organization (WHO). 2006. Extensively drug-resistant tuberculosis (XDR.TB): recommendations for prevention and control. Wkly Epidemiol Rec 81:430\u2013432.","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762011000500002"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004540"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1390-1394.2005"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.09.026"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2003.02171.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02180-07"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2004.12.003"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.08.021"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.04221-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.04221-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:03:26Z","timestamp":1645484606000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.04221-14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1128\/AAC.04221-14"],"URL":"https:\/\/doi.org\/10.1128\/aac.04221-14","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"2014-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-01-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}