{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:32:03Z","timestamp":1789810323926,"version":"4.0.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Mucosal biofilm-related fungal infections are very common, and the incidence of recurrent oral and vulvovaginal candidiasis is significant. As resistance to azoles (the preferred treatment) is occurring, we aimed at identifying compounds that increase the activity of miconazole against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Candida albicans<\/jats:named-content>\n            biofilms. We screened 1,600 compounds of a drug-repositioning library in combination with a subinhibitory concentration of miconazole. Synergy between the best identified potentiators and miconazole was characterized by checkerboard analyses and fractional inhibitory concentration indices. Hexachlorophene, pyrvinium pamoate, and artesunate act synergistically with miconazole in affecting\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            biofilms. Synergy was most pronounced for artesunate and structural homologues thereof. No synergistic effect could be observed between artesunate and fluconazole, caspofungin, or amphotericin B. Our data reveal enhancement of the antibiofilm activity of miconazole by artesunate, pointing to potential combination therapy consisting of miconazole and artesunate to treat\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            biofilm-related infections.\n          <\/jats:p>","DOI":"10.1128\/aac.04229-14","type":"journal-article","created":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T04:50:09Z","timestamp":1415076609000},"page":"421-426","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Artemisinins, New Miconazole Potentiators Resulting in Increased Activity against Candida albicans Biofilms"],"prefix":"10.1128","volume":"59","author":[{"given":"Kaat","family":"De Cremer","sequence":"first","affiliation":[{"name":"Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium"},{"name":"Department of Plant Systems Biology, VIB, Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Lanckacker","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanne L.","family":"Cools","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijke","family":"Bax","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrijn","family":"De Brucker","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Cos","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno P. A.","family":"Cammue","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium"},{"name":"Department of Plant Systems Biology, VIB, Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Thevissen","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/528521"},{"key":"e_1_3_3_3_2","first-page":"107","article-title":"Multidrug tolerance of biofilms and persister cells","volume":"322","author":"Lewis K","year":"2008","unstructured":"Lewis K. 2008. Multidrug tolerance of biofilms and persister cells. Curr Top Microbiol Immunol 322:107\u2013131.","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70266-4"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033705"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2011.585606"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.071746-0"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407673-0.00002-3"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2011.07.004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1273-3"},{"key":"e_1_3_3_11_2","first-page":"875","article-title":"Prophylactic strategies in recurrent vulvovaginal candidiasis: a 2-year study testing a phytonutrient vs itraconazole","volume":"27","author":"Chopra V","year":"2013","unstructured":"Chopra V, Marotta F, Kumari A, Bishier MP, He F, Zerbinati N, Agarwal C, Naito Y, Tomella C, Sharma ASU. 2013. Prophylactic strategies in recurrent vulvovaginal candidiasis: a 2-year study testing a phytonutrient vs itraconazole. J Biol Regul Homeost Agents 27:875\u2013882.","journal-title":"J Biol Regul Homeost Agents"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31827307b2"},{"key":"e_1_3_3_13_2","first-page":"379","article-title":"Oral candidosis: characterization of a sample of recurrent infections and study of resistance determinants","volume":"34","author":"Gall\u00e8 F","year":"2011","unstructured":"Gall\u00e8 F, Sanguinetti M, Colella G, Di Onofrio V, Torelli R, Rossano F, Liguori G. 2011. Oral candidosis: characterization of a sample of recurrent infections and study of resistance determinants. New Microbiol 34:379\u2013389.","journal-title":"New Microbiol"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-011-9460-4"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2012.12.001"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.2147\/HIV.S6660"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1310\/T7A7-1E63-2KA0-JKWD"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.101"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.13.189"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2011.44"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt449"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq530"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00294-13"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00680-13"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-013-0639-0"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.31"},{"key":"e_1_3_3_27_2","first-page":"1","article-title":"Repositioning drugs and biologics: retargeting old\/existing drugs for potential new therapeutic applications","volume":"4","author":"Sekhon BS","year":"2013","unstructured":"Sekhon BS. 2013. Repositioning drugs and biologics: retargeting old\/existing drugs for potential new therapeutic applications. J Pharm Educ Res 4:1\u201315.","journal-title":"J Pharm Educ Res"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874437001105010029"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/134.3.717"},{"key":"e_1_3_3_30_2","unstructured":"Reference deleted."},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2013.12.011"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04475.x"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01606.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg301"},{"key":"e_1_3_3_35_2","first-page":"439","article-title":"Review of the toxicity of hexachlorophene, including its neurotoxicity","volume":"13","author":"Kimbrough RD","year":"1973","unstructured":"Kimbrough RD. 1973. Review of the toxicity of hexachlorophene, including its neurotoxicity. J Clin Pharmacol 13:439\u2013444.","journal-title":"J Clin Pharmacol"},{"key":"e_1_3_3_36_2","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1002\/cpt1976196802","article-title":"Absorption of pyrvinium pamoate","volume":"19","author":"Smith TC","year":"1976","unstructured":"Smith TC, Kinkel AW, Gryczko CM, Goulet JR. 1976. Absorption of pyrvinium pamoate. Clin Pharmacol Ther 19:802\u2013806.","journal-title":"Clin Pharmacol Ther"},{"key":"e_1_3_3_37_2","volume-title":"Guidelines for the treatment of malaria","author":"World Health Organization","year":"2010","unstructured":"World Health Organization. 2010. Guidelines for the treatment of malaria, 2nd ed. WHO, Geneva, Switzerland.","edition":"2"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15031705"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2013.07.005"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009582"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2013.10.007"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0010036"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2010.00706.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00095.x"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq019"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00280-11"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.04229-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.04229-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:58:40Z","timestamp":1645484320000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.04229-14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1128\/AAC.04229-14"],"URL":"https:\/\/doi.org\/10.1128\/aac.04229-14","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1]]},"assertion":[{"value":"2014-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-10-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}