{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:29:37Z","timestamp":1792114177233,"version":"4.4.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycoplasma pneumoniae<\/jats:named-content>\n            is a major pathogen causing community-acquired pneumoniae (CAP), which is generally treated with macrolides. In recent years, however, although macrolide-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            has been reported frequently, particularly in China, very little is known about the prevalence of macrolide-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            infection in adults. In this study, we survey the macrolide-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            in adults in Zhejiang province and characterize the mechanisms of resistance to macrolide. Six hundred fifty throat swab samples were collected from adult patients with CAP from January 2012 to August 2014. These samples were assayed by nested PCR and then cultivated for\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            . All isolates were sequenced to determine the mutation in domain V of the 23S rRNA gene. The activities of 10 antibiotics against macrolide-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            isolates were also investigated\n            <jats:italic>in vitro<\/jats:italic>\n            . Moreover, restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 50 resistant strains. One hundred percent (71\/71) of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            strains isolated from adults with CAP were resistant to erythromycin (MIC = 128 to &gt;256 \u03bcg\/ml), clarithromycin (MIC = 128 to &gt;256 \u03bcg\/ml), and azithromycin (MIC = 32 to &gt;64 \u03bcg\/ml). Furthermore, all macrolide-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            strains identified had an A2063G mutation in domain V of the 23S rRNA gene. Forty-six resistant strains (92.0%) were classified into type I strain on the basis of P1 gene PCR-RFLP analysis. According to these findings, it is suggested that macrolide-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            infection is very prevalence among adults in Zhejiang province. Thus, there is necessary to perform the epidemiological monitoring of macrolide-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            in the future.\n          <\/jats:p>","DOI":"10.1128\/aac.04308-14","type":"journal-article","created":{"date-parts":[[2014,12,2]],"date-time":"2014-12-02T04:56:55Z","timestamp":1417496215000},"page":"1048-1051","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China"],"prefix":"10.1128","volume":"59","author":[{"given":"Zibo","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangzhi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangjun","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Zhuji People's Hospital, Zhuji, Zhejiang, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changwang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Zhuji People's Hospital, Zhuji, Zhejiang, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of 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