{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:45:54Z","timestamp":1791189954102,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Staphylococcal enterotoxin B (SEB) is a major virulence factor for staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T lymphocytic population, releasing proinflammatory cytokines. Blocking SEB-initiated toxicity may be an effective strategy for treating TSS. Using a process known as systematic evolution of ligands by exponential enrichment (SELEX), we identified an aptamer that can antagonize SEB with nanomolar binding affinity (\n            <jats:italic>\n              K\n              <jats:sub>d<\/jats:sub>\n            <\/jats:italic>\n            = 64 nM). The aptamer antagonist effectively inhibits SEB-mediated proliferation and cytokine secretion in human peripheral blood mononuclear cells. Moreover, a PEGylated aptamer antagonist significantly reduced mortality in a \u201cdouble-hit\u201d mouse model of SEB-induced TSS, established via sensitization with\n            <jats:sc>d<\/jats:sc>\n            -galactosamine followed by SEB challenge. Therefore, our novel aptamer antagonist may offer potential therapeutic efficacy against SEB-mediated TSS.\n          <\/jats:p>","DOI":"10.1128\/aac.04414-14","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T02:49:35Z","timestamp":1422326975000},"page":"2072-2077","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Neutralization of Staphylococcal Enterotoxin B by an Aptamer Antagonist"],"prefix":"10.1128","volume":"59","author":[{"given":"Kaiyu","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longjie","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyue","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopeng","family":"Lan","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00681.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2007.04.006"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830185.2013.823422"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/0960327112458941"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00526-12"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.875-884.2001"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit421"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2278-2281.2002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/74672"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1584"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309388200"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/355850a0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2200121"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/540451"},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"178","DOI":"10.2144\/02321dd04","article-title":"Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods","volume":"32","author":"Bruno JG","year":"2002","unstructured":"Bruno JG, Kiel JL. 2002. 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