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Carotenoids are polyisoprenoids from plants, algae, and some bacteria, and they are biosynthesized by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium falciparum<\/jats:named-content>\n            but not by mammalian cells. Biochemical and reverse genetics approaches were applied to demonstrate that phytoene synthase (PSY) is a key enzyme for carotenoid biosynthesis in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. falciparum<\/jats:named-content>\n            and is essential for intraerythrocytic growth. The known PSY inhibitor squalestatin reduces biosynthesis of phytoene and kills parasites during the intraerythrocytic cycle. PSY-overexpressing parasites showed increased biosynthesis of phytoene and its derived product phytofluene and presented a squalestatin-resistant phenotype, suggesting that this enzyme is the primary target of action of this drug in the parasite.\n          <\/jats:p>","DOI":"10.1128\/aac.04500-14","type":"journal-article","created":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T02:56:01Z","timestamp":1426560961000},"page":"3180-3188","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Squalestatin Is an Inhibitor of Carotenoid Biosynthesis in Plasmodium falciparum"],"prefix":"10.1128","volume":"59","author":[{"given":"Heloisa B.","family":"Gabriel","sequence":"first","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia F.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Em\u00edlia A.","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Wunderlich","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro M.","family":"Katzin","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro F.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"World malaria report","author":"WHO","year":"2013","unstructured":"WHO. 2013. 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