{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T06:09:07Z","timestamp":1655705347119},"reference-count":29,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Here, we report the first detection of a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            carbapenemase 2 (KPC-2)-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            strain belonging to sequence type 833 (ST833), collected in an Italian hospital from a patient coming from South America. Its\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            determinant was carried by a ColE1 plasmid, pKBuS13, that showed the Tn\n            <jats:italic>4401b<\/jats:italic>\n            ::\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            transposon inserted into the regulatory region of an Xer site-specific recombination locus. This interfered with the correct resolution of plasmid multimers into monomers, lowering plasmid stability and leading to overestimation of the number of plasmids harbored by a single host cell. Sequencing of the fragments adjacent to Tn\n            <jats:italic>4401b<\/jats:italic>\n            detected a region that did not have significant matches in databases other than the genome of a carbapenem-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            strain collected during the same year at a hospital in Boston. This is interesting in an epidemiologic context, as it suggests that despite the absence of\n            <jats:italic>tra<\/jats:italic>\n            genes and the instability under nonselective conditions, the circulation of pKBuS13 or of analogous plasmids might be wider than reported.\n          <\/jats:p>","DOI":"10.1128\/aac.04543-14","type":"journal-article","created":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T03:01:14Z","timestamp":1434423674000},"page":"5226-5231","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["pKBuS13, a KPC-2-Encoding Plasmid from Klebsiella pneumoniae Sequence Type 833, Carrying Tn\n            <i>4401b<\/i>\n            Inserted into an Xer Site-Specific Recombination Locus"],"prefix":"10.1128","volume":"59","author":[{"given":"Luigi","family":"Garbari","sequence":"first","affiliation":[{"name":"Department of Life Sciences, University of Trieste, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Busetti","sequence":"additional","affiliation":[{"name":"Institute for Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucilla","family":"Dolzani","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Petix","sequence":"additional","affiliation":[{"name":"Institute for Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Knezevich","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Microbiology CS, Azienda Ospedaliero-Universitaria Ospedali Riuniti, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaela","family":"Bressan","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizia","family":"Gionechetti","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico A.","family":"Tonin","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Lagatolla","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70190-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01783-10"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03600.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00126-09"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01818-09"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq431"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01451-07"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01773-09"},{"issue":"22","key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"pii: 20489","DOI":"10.2807\/ese.18.22.20489-en","article-title":"Epidemic diffusion of KPC carbapenemase-producing Klebsiella pneumoniae in Italy: results of the first countrywide survey","volume":"18","author":"Giani T","year":"2013","unstructured":"Giani T, Pini B, Arena F, Conte V, Bracco S, Migliavacca R, Pantosti A, Pagani L, Luzzaro F, Rossolini GM. 2013. 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