{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:43:27Z","timestamp":1790851407074,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Vibrio parahaemolyticus<\/jats:named-content>\n is an important causative agent of gastroenteritis, with the consumption of contaminated seafood being the major transmission route. Resistance to penicillin is common among\n V. parahaemolyticus<\/jats:named-content>\n strains, whereas cephalosporin resistance remains rare. In an attempt to assess the current prevalence and characteristics of antibiotic resistance of this pathogen in common food samples, a total of 54 (17% of the total samples)\n V. parahaemolyticus<\/jats:named-content>\n strains were isolated from 318 meat and seafood samples purchased from supermarkets and wet markets in Shenzhen, China, in 2013. These isolates exhibited high-level resistance to ampicillin, yet they were mostly susceptible to other antimicrobials, except for two that were resistant to extended-spectrum cephalosporins. The \u03b2-lactamase gene\n bla<\/jats:italic>\n PER-1<\/jats:sub>\n was detectable in one strain,\n V. parahaemolyticus<\/jats:named-content>\n V43, which was resistant to both third- and fourth-generation cephalosporins. Compared to other\n bla<\/jats:italic>\n PER-1<\/jats:sub>\n -positive\n V. parahaemolyticus<\/jats:named-content>\n strains reported in our previous studies, strain V43 was found to harbor an \u223c200-kb conjugative plasmid carrying genes that were different from the antimicrobial resistance genes reported from the previous studies. The \u03b2-lactamase gene\n bla<\/jats:italic>\n CMY-2<\/jats:sub>\n was detectable for the first time in another\n V. parahaemolyticus<\/jats:named-content>\n isolate, V4, which was resistant to third-generation cephalosporins. This\n bla<\/jats:italic>\n CMY-2<\/jats:sub>\n gene was shown to be located in an \u223c150-kb IncA\/C-type conjugative plasmid with a genetic structure consisting of\n traB-traV-traA<\/jats:italic>\n -IS\n Ecp1-bla<\/jats:italic>\n CMY-2<\/jats:sub>\n -\n blc-sugE-encR-orf1-orf2-orf3-orf4-dsbC-traC<\/jats:italic>\n , which is identical to that of other IncA\/C conjugative plasmids in\n Enterobacteriaceae<\/jats:named-content>\n , albeit with a different size. These findings indicate that the transmission of extended-spectrum-\u03b2-lactamase (ESBL) and AmpC \u03b2-lactamase genes via conjugative plasmids can mediate the development of extended-spectrum cephalosporin resistance in\n V. parahaemolyticus<\/jats:named-content>\n , thereby posing a potential threat to public health.\n <\/jats:p>","DOI":"10.1128\/aac.05008-14","type":"journal-article","created":{"date-parts":[[2015,4,28]],"date-time":"2015-04-28T01:51:47Z","timestamp":1430185907000},"page":"4106-4111","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["First Detection of AmpC \u03b2-Lactamase\n bla<\/i>\n CMY-2<\/sub>\n on a Conjugative IncA\/C Plasmid in a Vibrio parahaemolyticus Isolate of Food Origin"],"prefix":"10.1128","volume":"59","author":[{"given":"Ruichao","family":"Li","sequence":"first","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dachuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaichao","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5472-8337","authenticated-orcid":false,"given":"Marcus Ho Yin","family":"Wong","sequence":"additional","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People's Republic of China"},{"name":"State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.12.001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.10.018"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03792-12"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2013.04.017"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2013.08.014"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822011000400032"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01116-07"},{"key":"e_1_3_3_9_2","first-page":"53","article-title":"Susceptibility to antibiotics of Vibrio spp. and Photobacterium damsela ssp. piscicida strains isolated from Italian aquaculture farms","volume":"34","author":"Lagan\u00e0 P","year":"2011","unstructured":"Lagan\u00e0 P, Caruso G, Minutoli E, Zaccone R, Santi D. 2011. Susceptibility to antibiotics of Vibrio spp. and Photobacterium damsela ssp. piscicida strains isolated from Italian aquaculture farms. New Microbiol 34:53\u201363.","journal-title":"New Microbiol"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12258"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00385-12"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2010.06.013"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.11.034"},{"key":"e_1_3_3_14_2","volume-title":"CLSI document M45-P","author":"CLSI","year":"2005","unstructured":"CLSI. 2005. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; proposed guideline. CLSI document M45-P. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_15_2","volume-title":"CLSI document M100-S23","author":"CLSI","year":"2014","unstructured":"CLSI. 2014. Performance standards for antimicrobial susceptibility testing; 23rd informational supplement. CLSI document M100-S23. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"23"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp498"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2007.01.005"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku011"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-9"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00055-09"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12192"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00753-08"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00036-08"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.8.2845-2852.2004"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl416"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.05008-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.05008-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:56:46Z","timestamp":1645484206000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.05008-14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7]]},"references-count":25,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1128\/AAC.05008-14"],"URL":"https:\/\/doi.org\/10.1128\/aac.05008-14","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7]]},"assertion":[{"value":"2014-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-04-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-06-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}