{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T21:29:46Z","timestamp":1778707786159,"version":"3.51.4"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The EGD Murray collection consists of approximately 500 clinical bacterial isolates, mainly\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            , isolated from around the world between 1917 and 1949. A number of these \u201cMurray\u201d isolates have subsequently been identified as\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            . Antimicrobial susceptibility testing of these isolates showed that over 30% were resistant to penicillins due to the presence of diverse\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            \u03b2-lactamase genes. Analysis of susceptibility to skin antiseptics and triclosan showed that while the Murray isolates displayed a range of MIC\/minimal bactericidal concentration (MBC) values, the mean MIC value was lower than that for more modern\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            isolates tested. All Murray isolates contained the cation efflux gene\n            <jats:italic>cepA<\/jats:italic>\n            , which is involved in disinfectant resistance, but those that were more susceptible to chlorhexidine were found to have a 9- or 18-bp insertion in this gene. Susceptibility to other disinfectants, e.g., H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            , in the Murray isolates was comparable to that in modern\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            isolates. The Murray isolates were also less virulent in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Galleria<\/jats:named-content>\n            and had a different complement of putative virulence factors than the modern isolates, with the exception of an isolate related to the modern lineage CC23. More of the modern isolates (41% compared to 8%) are classified as good\/very good biofilm formers, but there was overlap in the two populations. This study demonstrated that a significant proportion of the Murray\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella<\/jats:named-content>\n            isolates were resistant to penicillins before their routine use. This collection of pre-antibiotic era isolates may provide significant insights into adaptation in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            in relation to biocide susceptibility.\n          <\/jats:p>","DOI":"10.1128\/aac.05009-14","type":"journal-article","created":{"date-parts":[[2015,4,21]],"date-time":"2015-04-21T02:31:49Z","timestamp":1429583509000},"page":"3966-3972","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Characterization of Pre-Antibiotic Era Klebsiella pneumoniae Isolates with Respect to Antibiotic\/Disinfectant Susceptibility and Virulence in Galleria mellonella"],"prefix":"10.1128","volume":"59","author":[{"given":"Matthew E.","family":"Wand","sequence":"first","affiliation":[{"name":"Public Health England, Microbiology Services Division, Salisbury, Wiltshire, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate S.","family":"Baker","sequence":"additional","affiliation":[{"name":"Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Benthall","sequence":"additional","affiliation":[{"name":"Public Health England, Microbiology Services Division, Salisbury, Wiltshire, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"McGregor","sequence":"additional","affiliation":[{"name":"National Collection of Type Cultures, Culture Collections, Public Health England, Salisbury, Wiltshire, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W. I.","family":"McCowen","sequence":"additional","affiliation":[{"name":"Public Health England, Microbiology Services Division, Salisbury, Wiltshire, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Deheer-Graham","sequence":"additional","affiliation":[{"name":"National Collection of Type Cultures, Culture Collections, Public Health England, Salisbury, Wiltshire, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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