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The presence of two<jats:italic>erg11<\/jats:italic>(<jats:italic>cyp51<\/jats:italic>) genes in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus<\/jats:named-content>spp. is hypothesized to account for the inherent resistance of this mold to the triazole fluconazole (FLC). Recently, an<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. fumigatus<\/jats:named-content>null mutant of a transcriptional regulator in the sterol regulatory element binding protein (SREBP) family, the \u0394<jats:italic>srbA<\/jats:italic>strain, was found to have increased susceptibility to FLC and voriconazole (VCZ). In this study, we examined the mechanism engendering the observed increase in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. fumigatus<\/jats:named-content>triazole susceptibility in the absence of SrbA. We observed a significant reduction in the<jats:italic>erg11A<\/jats:italic>transcript in the \u0394<jats:italic>srbA<\/jats:italic>strain in response to FLC and VCZ. Transcript levels of<jats:italic>erg11B<\/jats:italic>were also reduced but not to the extent of<jats:italic>erg11A<\/jats:italic>. Interestingly,<jats:italic>erg11A<\/jats:italic>transcript levels increased upon extended VCZ, but not FLC, exposure. Construction of an<jats:italic>erg11A<\/jats:italic>conditional expression strain in the \u0394<jats:italic>srbA<\/jats:italic>strain was able to restore<jats:italic>erg11A<\/jats:italic>transcript levels and, consequently, wild-type MICs to the triazole FLC. The VCZ MIC was also partially restored upon increased<jats:italic>erg11A<\/jats:italic>transcript levels; however, total ergosterol levels remained significantly reduced compared to those of the wild type. Induction of the<jats:italic>erg11A<\/jats:italic>conditional strain did not restore the hypoxia growth defect of the \u0394<jats:italic>srbA<\/jats:italic>strain. Taken together, our results demonstrate a critical role for SrbA-mediated regulation of ergosterol biosynthesis and triazole drug interactions in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. fumigatus<\/jats:named-content>that may have clinical importance.<\/jats:p>","DOI":"10.1128\/aac.05027-11","type":"journal-article","created":{"date-parts":[[2011,10,18]],"date-time":"2011-10-18T05:22:42Z","timestamp":1318915362000},"page":"248-257","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["SREBP-Dependent Triazole Susceptibility in Aspergillus fumigatus Is Mediated through Direct Transcriptional Regulation of<i>erg11A<\/i>(<i>cyp51A<\/i>)"],"prefix":"10.1128","volume":"56","author":[{"given":"Sara J.","family":"Blosser","sequence":"first","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Cramer","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00517-11"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.512926"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2007.11.005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"657","DOI":"10.2165\/00003495-199754050-00002","article-title":"Antifungal resistance trends towards the year 2000. 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