{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:47:06Z","timestamp":1791150426614,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n A carbapenem-resistant\n Serratia marcescens<\/jats:named-content>\n strain, 10mdr148, was identified in a Japanese hospital in 2010. The carbapenem resistance of this strain was attributed to the production of a novel metallo-\u03b2-lactamase (MBL), named SMB-1 (\n \n S<\/jats:underline>\n erratia\n <\/jats:named-content>\n m<\/jats:underline>\n etallo-\n \u03b2<\/jats:underline>\n -lactamase). SMB-1 possessed a zinc binding motif, H(Q)XHXDH (residues 116 to 121), H196, and H263 and was categorized as a member of subclass B3 MBL. SMB-1 has 75% amino acid identity with the most closely related MBL, AMO1, of uncultured bacterium, recently identified through the metagenomic analysis of apple orchard soil. The introduction of\n bla<\/jats:italic>\n SMB-1<\/jats:sub>\n into\n Escherichia coli<\/jats:named-content>\n conferred resistance to a variety of \u03b2-lactam antibiotics, penicillins, cephalosporins, and carbapenems, but not aztreonam, a resistance pattern consistent with those of other MBLs. SMB-1 demonstrated high\n k<\/jats:italic>\n cat<\/jats:sub>\n values of >500 s\n \u22121<\/jats:sup>\n for carbapenems, resulting in the highest hydrolyzing efficiency (\n k<\/jats:italic>\n cat<\/jats:sub>\n \/\n \n K\n m<\/jats:sub>\n <\/jats:italic>\n ) among the agents tested. The hydrolyzing activity of SMB-1 was well inhibited by chelating agents. The\n bla<\/jats:italic>\n SMB-1<\/jats:sub>\n gene was located on the chromosome of\n S. marcescens<\/jats:named-content>\n strain 10mdr148 and at the 3\u2032 end of the IS\n CR1<\/jats:italic>\n element in complex with a typical class 1 integron carrying\n aac<\/jats:italic>\n (\n 6\u2032<\/jats:italic>\n )\n -Ib<\/jats:italic>\n and\n catB3<\/jats:italic>\n gene cassettes. Downstream of\n bla<\/jats:italic>\n SMB-1<\/jats:sub>\n , the second copy of the 3\u2032conserved segment and IS\n CR1<\/jats:italic>\n were found. To our knowledge, this is the first subclass B3 MBL gene associated with an IS\n CR1<\/jats:italic>\n element identified in an\n Enterobacteriaceae<\/jats:italic>\n clinical isolate. A variety of antibiotic resistance genes embedded with IS\n CR1<\/jats:italic>\n have been widely spread among\n Enterobacteriaceae<\/jats:italic>\n clinical isolates, thus the further dissemination of\n bla<\/jats:italic>\n SMB-1<\/jats:sub>\n mediated by IS\n CR1<\/jats:italic>\n transposition activity may become a future concern.\n <\/jats:p>","DOI":"10.1128\/aac.05045-11","type":"journal-article","created":{"date-parts":[[2011,8,30]],"date-time":"2011-08-30T01:47:57Z","timestamp":1314668877000},"page":"5143-5149","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":92,"title":["SMB-1, a Novel Subclass B3 Metallo-\u03b2-Lactamase, Associated with IS\n CR1<\/i>\n and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate"],"prefix":"10.1128","volume":"55","author":[{"given":"Jun-ichi","family":"Wachino","sequence":"first","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Prevention, Kobe University Hospital, 7-5-2 Chuo-ku, Kobe, Hyogo 650-0017, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunikazu","family":"Yamane","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"},{"name":"Department of Public Health, Kawasaki Medical University, 577 Matsushima, Kurashiki, Okayama 701-0192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satowa","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Matsui","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Yamagishi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Tsutsui","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshifumi","family":"Konda","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keigo","family":"Shibayama","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshichika","family":"Arakawa","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan"},{"name":"Department of Bacteriology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1878-1886.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.09.006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01009-09"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4654-4661.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq054"},{"key":"e_1_3_2_7_2","volume-title":"Approved standard, 8th ed. 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