{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:03:20Z","timestamp":1791299000378,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The epidemiology of carbapenemases worldwide is showing that OXA-48 variants are becoming the predominant carbapenemase type in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            in many countries. However, not all OXA-48 variants possess significant activity toward carbapenems (e.g., OXA-163). Two\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Serratia marcescens<\/jats:named-content>\n            isolates with resistance either to carbapenems or to extended-spectrum cephalosporins were successively recovered from the same patient. A genomic comparison using pulsed-field gel electrophoresis and automated Rep-PCR typing identified a 97.8% similarity between the two isolates. Both strains were resistant to penicillins and first-generation cephalosporins. The first isolate was susceptible to expanded-spectrum cephalosporins, was resistant to carbapenems, and had a significant carbapenemase activity (positive Carba NP test) related to the expression of OXA-48. The second isolate was resistant to expanded-spectrum cephalosporins, was susceptible to carbapenems, and did not express a significant imipenemase activity, (negative for the Carba NP test) despite possessing a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-48<\/jats:sub>\n            -type gene. Sequencing identified a novel OXA-48-type \u03b2-lactamase, OXA-405, with a four-amino-acid deletion compared to OXA-48. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-405<\/jats:sub>\n            gene was located on a ca. 46-kb plasmid identical to the prototype IncL\/M\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-48<\/jats:sub>\n            -carrying plasmid except for a ca. 16.4-kb deletion in the\n            <jats:italic>tra<\/jats:italic>\n            operon, leading to the suppression of self-conjugation properties. Biochemical analysis showed that OXA-405 has clavulanic acid-inhibited activity toward expanded-spectrum activity without significant imipenemase activity. This is the first identification of a successive switch of catalytic activity in OXA-48-like \u03b2-lactamases, suggesting their plasticity. Therefore, this report suggests that the first-line screening of carbapenemase producers in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            may be based on the biochemical detection of carbapenemase activity in clinical settings.\n          <\/jats:p>","DOI":"10.1128\/aac.05058-14","type":"journal-article","created":{"date-parts":[[2015,4,14]],"date-time":"2015-04-14T02:22:21Z","timestamp":1428978141000},"page":"3823-3828","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["Genetic and Biochemical Characterization of OXA-405, an OXA-48-Type Extended-Spectrum \u03b2-Lactamase without Significant Carbapenemase Activity"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7384","authenticated-orcid":false,"given":"Laurent","family":"Dortet","sequence":"first","affiliation":[{"name":"INSERM U 914, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"Faculty of Medicine, South-Paris University, Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Bic\u00eatre Hospital, Assistance Publique\/H\u00f4pitaux de Paris, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saoussen","family":"Oueslati","sequence":"additional","affiliation":[{"name":"INSERM U 914, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katy","family":"Jeannot","sequence":"additional","affiliation":[{"name":"Associated National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"Besan\u00e7on Hospital, Microbiology Laboratory, Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Tand\u00e9","sequence":"additional","affiliation":[{"name":"Brest Hospital, Microbiology Laboratory, Brest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"INSERM U 914, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"Faculty of Medicine, South-Paris University, Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Bic\u00eatre Hospital, Assistance Publique\/H\u00f4pitaux de Paris, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"INSERM U 914, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"Medical and Microbiology Unit, Department of Medicine, University Fribourg, Fribourg, Switzerland"},{"name":"HFR-H\u00f4pital Cantonal, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01512-08"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00856-09"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01456.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks121"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.15-22.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2012.702343"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06710-11"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00022-11"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00481-11"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01034-12"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.11.007"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks383"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12142"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02510-13"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1809.120355"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.05.021"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.071340-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(84)90063-5"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05289-11"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.04.010"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110530108"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi501547k"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.9.2598-2603.2001"}],"container-title":["Antimicrobial 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