{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:47:31Z","timestamp":1791226051490,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Aspergillus fumigatus<\/jats:named-content>\n is the most frequent fungus found in the sputum of cystic fibrosis (CF) subjects. Itraconazole is prescribed for allergic bronchopulmonary aspergillosis (ABPA) or\n Aspergillus<\/jats:named-content>\n bronchitis in CF subjects. We hypothesized that\n A. fumigatus<\/jats:named-content>\n isolates in the sputum of CF subjects with previous exposure to itraconazole was associated with higher prevalence of azole resistance. From June 2010 to April 2011, sputum samples from adult CF subjects at Cochin University Hospital (France) were examined systematically for the detection of\n A. fumigatus<\/jats:named-content>\n . MICs of\n A. fumigatus<\/jats:named-content>\n isolates against azoles were screened using Etest, and reduced susceptibility to azoles was confirmed using the CLSI broth microdilution method.\n A. fumigatus<\/jats:named-content>\n was isolated from the sputum of 131\/249 (52.6%) adult CF subjects, and 47\/131 (35.9%) subjects had received previous treatment with itraconazole. Reduced\n A. fumigatus<\/jats:named-content>\n susceptibility to itraconazole (MIC, \u22652 mg\/liter) was confirmed in 6\/131 (4.6%) subjects. All 6 isolates also had reduced susceptibility to posaconazole (MIC, \u22650.5 mg\/liter), and 3\/6 isolates had reduced susceptibility to voriconazole (MIC, \u22652 mg\/liter). Mutations in the\n cyp51A<\/jats:italic>\n gene were detected at positions previously implicated to cause resistance in 5 isolates. Azole-resistant\n A. fumigatus<\/jats:named-content>\n isolates were found in 5\/25 (20%) subjects exposed to itraconazole within the previous 3 years. High rates of azole-resistant\n A. fumigatus<\/jats:named-content>\n isolates were present in adult CF subjects and were associated with recent itraconazole exposure. Although the clinical implications of these findings will require further studies, the cautious use of itraconazole in adult CF subjects can be recommended.\n <\/jats:p>","DOI":"10.1128\/aac.05077-11","type":"journal-article","created":{"date-parts":[[2011,11,30]],"date-time":"2011-11-30T02:31:59Z","timestamp":1322620319000},"page":"869-874","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":169,"title":["High Prevalence of Azole-Resistant Aspergillus fumigatus in Adults with Cystic Fibrosis Exposed to Itraconazole"],"prefix":"10.1128","volume":"56","author":[{"given":"Pierre-R\u00e9gis","family":"Burgel","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Pneumologie, H\u00f4pital Cochin, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Th\u00e9r\u00e8se","family":"Baixench","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Cochin, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u00ebl","family":"Amsellem","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Cochin, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Audureau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service d'Epid\u00e9miologie et Biostatistiques, H\u00f4pital H\u00f4tel-Dieu, AP-HP, Paris, 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France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Dupouy-Camet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Cochin, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Dusser","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Pneumologie, H\u00f4pital Cochin, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corn\u00e9 H.","family":"Klaassen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands"},{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Hubert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Pneumologie, H\u00f4pital Cochin, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Paugam","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Cochin, AP-HP, Paris, 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