{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T21:45:40Z","timestamp":1782423940497,"version":"3.54.5"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The need to identify improved therapy against cystic echinococcosis (CE) has motivated pharmacology-based research. The comparative pharmacological performances of the benzimidazole compounds flubendazole (FLBZ) and albendazole (ABZ) were addressed here. The goals of the work were as follows: (i) to evaluate the<jats:italic>ex vivo<\/jats:italic>activities of FLBZ, ABZ, and their respective metabolites against<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Echinococcus granulosus<\/jats:named-content>protoscoleces, (ii) to compare the plasma and cyst disposition kinetics for the two drugs in infected mice, and (iii) to compare the clinical efficacies of FLBZ and ABZ against CE in mice. For the<jats:italic>ex vivo<\/jats:italic>study,<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. granulosus<\/jats:named-content>protoscoleces were incubated with FLBZ, reduced FLBZ (R-FLBZ), ABZ, and ABZ-sulfoxide (ABZSO) (10 nmol\/ml). Protoscolex viability was monitored by the methylene blue exclusion test and scanning electron microscopy (SEM). For the pharmacokinetic study, BALB\/c mice with CE were allocated to two different groups and orally treated with either FLBZ or ABZ (5 mg\/kg of body weight), both formulated as a cyclodextrin-based solution. Blood and cyst samples were taken up to 12 h posttreatment and analyzed by high-performance liquid chromatography (HPLC). For the efficacy study, CE-infected BALB\/c mice were divided into three groups: the unmedicated control group and the FLBZ- and ABZ-treated groups. Oral treatments were performed twice a day during 25 days. After treatment, all animals were killed and the weight of the cysts was recorded. Loss of protoscolex viability was observed after drug incubation. FLBZ was detected in plasma (area under the concentration-versus-time curve [AUC] = 1.8 \u03bcg\u00b7h\/ml) and cysts (AUC = 0.3 \u03bcg\u00b7h\/g) collected from treated infected animals. Conversely, ABZSO was the only active molecule measured in plasma (AUC = 4.4 \u03bcg\u00b7h\/ml) and cysts (AUC = 1.5 \u03bcg\u00b7h\/g) after ABZ treatment. FLBZ induced a 90% reduction in cyst weight in comparison to those collected from untreated control mice (<jats:italic>P<\/jats:italic>&lt; 0.05). However, no differences in cyst weight were observed between the ABZ-treated (8.2 g) and unmedicated control (10.5 g) groups. Due to these results, we consider flubendazole to have great potential to become a drug of choice in the treatment of cystic echinococcosis.<\/jats:p>","DOI":"10.1128\/aac.05105-11","type":"journal-article","created":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T08:18:33Z","timestamp":1316506713000},"page":"5861-5867","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Comparative Performances of Flubendazole and Albendazole in Cystic Echinococcosis:<i>Ex Vivo<\/i>Activity, Plasma\/Cyst Disposition, and Efficacy in Infected Mice"],"prefix":"10.1128","volume":"55","author":[{"given":"Laura","family":"Ceballos","sequence":"first","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Fisiopatolog\u00eda, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Campus Universitario, 7000 Tandil, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celina","family":"Elissondo","sequence":"additional","affiliation":[{"name":"Laboratorio de Zoonosis Parasitarias, Departamento de Biolog\u00eda, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3250, 7600 Mar del Plata, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"S\u00e1nchez Bruni","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Fisiopatolog\u00eda, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Campus Universitario, 7000 Tandil, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Denegri","sequence":"additional","affiliation":[{"name":"Laboratorio de Zoonosis Parasitarias, Departamento de Biolog\u00eda, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3250, 7600 Mar del Plata, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Lanusse","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Fisiopatolog\u00eda, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Campus Universitario, 7000 Tandil, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Fisiopatolog\u00eda, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Campus Universitario, 7000 Tandil, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.05.014"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2007.01.003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2885.1999.00194.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(96)90010-7"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931155"},{"key":"e_1_3_2_7_2","first-page":"85","article-title":"Preliminary kinetic data on flubendazole formulated as a hydroxypropyl-\u03b2-cyclodextrin aqueous solution for use in sheep","volume":"29","author":"Ceballos L.","year":"2006","unstructured":"CeballosL.. 2006. 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