{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T21:00:37Z","timestamp":1770411637264,"version":"3.49.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previous research has shown that 1% to 10% of sessile\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Candida albicans<\/jats:named-content>\n            cells survive treatment with high doses of miconazole (a fungicidal imidazole). In the present study, we investigated the involvement of sphingolipid biosynthetic intermediates in this survival. We observed that the\n            <jats:italic>LCB4<\/jats:italic>\n            gene, coding for the enzyme that catalyzes the phosphorylation of dihydrosphingosine and phytosphingosine, is important in governing the miconazole resistance of sessile\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Saccharomyces cerevisiae<\/jats:named-content>\n            and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            cells. The addition of 10 nM phytosphingosine-1-phosphate (PHS-1-P) drastically reduced the intracellular miconazole concentration and significantly increased the miconazole resistance of a hypersusceptible\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            heterozygous\n            <jats:italic>LCB4\/lcb4<\/jats:italic>\n            mutant, indicating a protective effect of PHS-1-P against miconazole-induced cell death in sessile cells. At this concentration of PHS-1-P, we did not observe any effect on the fluidity of the cytoplasmic membrane. The protective effect of PHS-1-P was not observed when the efflux pumps were inhibited or when tested in a mutant without functional efflux systems. Also, the addition of PHS-1-P during miconazole treatment increased the expression levels of genes coding for efflux pumps, leading to the hypothesis that PHS-1-P acts as a signaling molecule and enhances the efflux of miconazole in sessile\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            cells.\n          <\/jats:p>","DOI":"10.1128\/aac.05106-11","type":"journal-article","created":{"date-parts":[[2012,2,22]],"date-time":"2012-02-22T02:37:05Z","timestamp":1329878225000},"page":"2290-2294","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Phytosphingosine-1-Phosphate Is a Signaling Molecule Involved in Miconazole Resistance in Sessile Candida albicans Cells"],"prefix":"10.1128","volume":"56","author":[{"given":"Davy","family":"Vandenbosch","sequence":"first","affiliation":[{"name":"Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bink","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, K. U. Leuven, Heverlee, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilmer","family":"Govaert","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, K. U. Leuven, Heverlee, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno P. A.","family":"Cammue","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, K. U. Leuven, Heverlee, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans J.","family":"Nelis","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Thevissen","sequence":"additional","affiliation":[{"name":"Centre of Microbial and Plant Genetics, K. U. Leuven, Heverlee, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Coenye","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.14.4638-4644.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1515\/BC.2002.169","article-title":"Lipid rafts in protein sorting and cell polarity in budding yeast Saccharomyces cerevisiae","volume":"383","author":"Bagnat M","year":"2002","unstructured":"BagnatM SimonsK. 2002. Lipid rafts in protein sorting and cell polarity in budding yeast Saccharomyces cerevisiae. Biol. Chem. 383:1475\u20131480.","journal-title":"Biol. 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