{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:48:20Z","timestamp":1790516900130,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Recent studies have demonstrated that some morphologically atypical\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus fumigatus<\/jats:named-content>\n            strains are different species belonging to the section\n            <jats:italic>Fumigati<\/jats:italic>\n            .\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus lentulus<\/jats:named-content>\n            , one of these sibling species, is increasingly reported in patients under corticosteroid treatment. MICs of most antifungals in clinical use are elevated against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. lentulus<\/jats:named-content>\n            , and it shows primary resistance to azole drugs. Two\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. lentulus<\/jats:named-content>\n            cytochrome P450 14-\u03b1 sterol demethylases, encoded by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. lentulus<\/jats:named-content>\n            c\n            <jats:italic>yp51<\/jats:italic>\n            A (Alc\n            <jats:italic>yp51A<\/jats:italic>\n            ) and Al\n            <jats:italic>cyp51B<\/jats:italic>\n            genes, were identified. Targeted\n            <jats:italic>cyp51A<\/jats:italic>\n            gene knockout in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. lentulus<\/jats:named-content>\n            showed that the intrinsic azole resistance of this species is\n            <jats:italic>cyp51A<\/jats:italic>\n            dependent. The \u0394\n            <jats:italic>cyp51A<\/jats:italic>\n            strain was morphologically indistinguishable from the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. lentulus<\/jats:named-content>\n            wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice. The heterologous expression of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. lentulus<\/jats:named-content>\n            <jats:italic>cyp51A<\/jats:italic>\n            was performed in an\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. fumigatus<\/jats:named-content>\n            <jats:italic>cyp51A<\/jats:italic>\n            -deficient strain, confirming that Cyp51A is responsible for the differences in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. lentulus<\/jats:named-content>\n            -azole drug interaction.\n          <\/jats:p>","DOI":"10.1128\/aac.05178-11","type":"journal-article","created":{"date-parts":[[2011,9,27]],"date-time":"2011-09-27T03:50:46Z","timestamp":1317095446000},"page":"5459-5468","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Role of Aspergillus lentulus 14-\u03b1 Sterol Demethylase (Cyp51A) in Azole Drug Susceptibility"],"prefix":"10.1128","volume":"55","author":[{"given":"Emilia","family":"Mellado","sequence":"first","affiliation":[{"name":"Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Alcazar-Fuoli","sequence":"additional","affiliation":[{"name":"Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, 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