{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:46:48Z","timestamp":1792457208150,"version":"4.5.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Fusarium<\/jats:named-content>\n (\n n<\/jats:italic>\n = 67) and\n Scedosporium<\/jats:named-content>\n (\n n<\/jats:italic>\n = 63) clinical isolates were tested by two reference broth microdilution (BMD) methods against a novel broad-spectrum (active against both yeasts and molds) antifungal, E1210, and comparator agents. E1210 inhibits the inositol acylation step in glycophosphatidylinositol (GPI) biosynthesis, resulting in defects in fungal cell wall biosynthesis. Five species complex organisms\/species of\n Fusarium<\/jats:named-content>\n (4 isolates unspeciated) and 28\n Scedosporium apiospermum<\/jats:named-content>\n , 7\n Scedosporium aurantiacum<\/jats:named-content>\n , and 28\n Scedosporium prolificans<\/jats:named-content>\n species were identified by molecular techniques. Comparator antifungal agents included anidulafungin, caspofungin, itraconazole, posaconazole, voriconazole, and amphotericin B. E1210 was highly active against all of the tested isolates, with minimum effective concentration (MEC)\/MIC\n 90<\/jats:sub>\n values (\u03bcg\/ml) for E1210, anidulafungin, caspofungin, itraconazole, posaconazole, voriconazole, and amphotericin B, respectively, for\n Fusarium<\/jats:named-content>\n of 0.12, >16, >16, >8, >8, 8, and 4 \u03bcg\/ml. E1210 was very potent against the\n Scedosporium<\/jats:named-content>\n spp. tested. The E1210 MEC\n 90<\/jats:sub>\n was 0.12 \u03bcg\/ml for\n S. apiospermum<\/jats:named-content>\n , but 1 to >8 \u03bcg\/ml for other tested agents. Against\n S. aurantiacum<\/jats:named-content>\n , the MEC\n 50<\/jats:sub>\n for E1210 was 0.06 \u03bcg\/ml versus 0.5 to >8 \u03bcg\/ml for the comparators. Against\n S. prolificans<\/jats:named-content>\n , the MEC\n 90<\/jats:sub>\n for E1210 was only 0.12 \u03bcg\/ml, compared to >4 \u03bcg\/ml for amphotericin B and >8 \u03bcg\/ml for itraconazole, posaconazole, and voriconazole. Both CLSI and EUCAST methods were highly concordant for E1210 and all comparator agents. The essential agreement (EA; \u00b12 doubling dilutions) was >93% for all comparisons, with the exception of posaconazole and\n F. oxysporum<\/jats:named-content>\n species complex (SC) (60%), posaconazole and\n S. aurantiacum<\/jats:named-content>\n (85.7%), and voriconazole and\n S. aurantiacum<\/jats:named-content>\n (85.7%). In conclusion, E1210 exhibited very potent and broad-spectrum antifungal activity against azole- and amphotericin B-resistant strains of\n Fusarium<\/jats:named-content>\n spp. and\n Scedosporium<\/jats:named-content>\n spp. Furthermore,\n in vitro<\/jats:italic>\n susceptibility testing of E1210 against isolates of\n Fusarium<\/jats:named-content>\n and\n Scedosporium<\/jats:named-content>\n may be accomplished using either of the CLSI or EUCAST BMD methods, each producing very similar results.\n <\/jats:p>","DOI":"10.1128\/aac.05414-11","type":"journal-article","created":{"date-parts":[[2011,11,15]],"date-time":"2011-11-15T14:06:06Z","timestamp":1321365966000},"page":"352-357","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":95,"title":["Activities of E1210 and Comparator Agents Tested by CLSI and EUCAST Broth Microdilution Methods against Fusarium and Scedosporium Species Identified Using Molecular Methods"],"prefix":"10.1128","volume":"56","author":[{"given":"Mariana","family":"Castanheira","sequence":"first","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick P.","family":"Duncanson","sequence":"additional","affiliation":[{"name":"Eisai, Inc., Woodcliff Lake, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Diekema","sequence":"additional","affiliation":[{"name":"University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Guarro","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald N.","family":"Jones","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"},{"name":"Tufts University School of Medicine, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Pfaller","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"},{"name":"University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn022"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01618-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01685-08"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/00365540600786564","article-title":"Scedosporium prolificans brain abscess in a patient with chronic granulomatous disease: successful combination therapy with voriconazole and terbinafine","volume":"39","author":"Bhat SV","year":"2007","unstructured":"BhatSV PatersonDL RinaldiMG VeldkampPJ. 2007. 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