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All M\u03b2Ls structurally characterized so far present a Cys or a Ser residue at position 221, which is critical for catalysis. GOB lactamases stand as an exception within this picture, possessing a Met residue in this location. We studied different mutants in this position, and we show that Met221 is essential for protein stability, most likely due to its involvement in a hydrophobic core. In contrast to other known M\u03b2Ls, residue 221 is not involved in metal binding or in catalysis in GOB enzymes, further highlighting the structural diversity of M\u03b2Ls. We also demonstrate the usefulness of protein periplasmic profiles to assess the contribution of protein stability to antibiotic resistance.<\/jats:p>","DOI":"10.1128\/aac.05418-11","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T05:13:08Z","timestamp":1326863588000},"page":"1769-1773","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>In Vivo<\/i>Impact of Met221 Substitution in GOB Metallo-\u03b2-Lactamase"],"prefix":"10.1128","volume":"56","author":[{"given":"Jorgelina","family":"Mor\u00e1n-Barrio","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica and Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR, CONICET-UNR), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda-Natalia","family":"Lisa","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica and Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR, CONICET-UNR), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro J.","family":"Vila","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica and Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR, CONICET-UNR), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2008.86"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2007.05.021"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00174.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/S0969-2126(96)00089-5","article-title":"Crystal structure of the wide-spectrum binuclear zinc beta-lactamase from Bacteroides fragilis","volume":"4","author":"Concha N","year":"1996","unstructured":"ConchaN RasmussenBA BushK HerzbergO. 1996. 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