{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T09:39:19Z","timestamp":1780047559624,"version":"3.53.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Despite efforts to reduce malaria morbidity and mortality, drug-resistant parasites continue to evade control strategies. Recently, emphasis has shifted away from control and toward regional elimination and global eradication of malaria. Such a campaign requires tools to monitor genetic changes in the parasite that could compromise the effectiveness of antimalarial drugs and undermine eradication programs. These tools must be fast, sensitive, unambiguous, and cost-effective to offer real-time reports of parasite drug susceptibility status across the globe. We have developed and validated a set of genotyping assays using high-resolution melting (HRM) analysis to detect molecular biomarkers associated with drug resistance across six genes in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium falciparum<\/jats:named-content>\n            . We improved on existing technical approaches by developing refinements and extensions of HRM, including the use of blocked probes (LunaProbes) and the mutant allele amplification bias (MAAB) technique. To validate the sensitivity and accuracy of our assays, we compared our findings to sequencing results in both culture-adapted lines and clinical isolates from Senegal. We demonstrate that our assays (i) identify both known and novel polymorphisms, (ii) detect multiple genotypes indicative of mixed infections, and (iii) distinguish between variants when multiple copies of a locus are present. These rapid and inexpensive assays can track drug resistance and detect emerging mutations in targeted genetic loci in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. falciparum<\/jats:named-content>\n            . They provide tools for monitoring molecular changes associated with changes in drug response across populations and for determining whether parasites present after drug treatment are the result of recrudescence or reinfection in clinical settings.\n          <\/jats:p>","DOI":"10.1128\/aac.05737-11","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T03:32:13Z","timestamp":1332214333000},"page":"2976-2986","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Rapid, Field-Deployable Method for Genotyping and Discovery of Single-Nucleotide Polymorphisms Associated with Drug Resistance in Plasmodium falciparum"],"prefix":"10.1128","volume":"56","author":[{"given":"Rachel","family":"Daniels","sequence":"first","affiliation":[{"name":"Broad Institute, Cambridge, Massachusetts, USA"},{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daouda","family":"Ndiaye","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Cheikh Anta Diop, Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikeal","family":"Wall","sequence":"additional","affiliation":[{"name":"Idaho Technology, Inc., Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"McKinney","sequence":"additional","affiliation":[{"name":"Idaho Technology, Inc., Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Papa Diogoye","family":"S\u00e9ne","sequence":"additional","affiliation":[{"name":"Broad Institute, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pardis C.","family":"Sabeti","sequence":"additional","affiliation":[{"name":"Broad Institute, Cambridge, Massachusetts, USA"},{"name":"Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA"},{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah K.","family":"Volkman","sequence":"additional","affiliation":[{"name":"Broad Institute, Cambridge, Massachusetts, USA"},{"name":"School for Nursing and Health Sciences, Simmons College, Boston, Massachusetts, USA"},{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souleymane","family":"Mboup","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Cheikh Anta Diop, Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dyann F.","family":"Wirth","sequence":"additional","affiliation":[{"name":"Broad Institute, Cambridge, Massachusetts, USA"},{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2009.05.013"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01144-06"},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.4269\/ajtmh.1994.50.193","article-title":"In vitro activity of pyrimethamine, cycloguanil, and other antimalarial drugs against African isolates and clones of Plasmodium falciparum","volume":"50","author":"Basco LK","year":"1994","unstructured":"BascoLK RamiliarisoaO Le BrasJ. 1994. 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