{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:58:34Z","timestamp":1762865914615},"reference-count":55,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Reduced susceptibility to glycopeptides in methicillin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            (MRSA) clinical isolates is considered a risk factor for failure of glycopeptide therapy. We compared the prevalences of MRSA isolates with reduced glycopeptide susceptibility in patients with versus without persistent or recurrent MRSA bloodstream infections. A retrospective cohort study at the University Hospital of Geneva identified 27 patients with persistent or recurrent clonally related MRSA bacteremic episodes over an 8-year period, which included 208 consecutive nosocomial MRSA bacteremic episodes. Vancomycin and teicoplanin MICs were determined by a modified macrodilution assay allowing improved detection of glycopeptide-intermediate MRSA isolates (GISA), characterized by elevated teicoplanin or\/and vancomycin MICs (\u22654 \u03bcg\/ml). For 16 patients (59%), their pretherapy and\/or posttherapy MRSA isolates showed elevated teicoplanin MICs, among which 10 (37%) concomitantly displayed elevated vancomycin MICs. In contrast, 11 other patients (41%) were persistently or recurrently infected with non-GISA isolates. In comparison, only 39 (22%) of 181 single isolates from patients with no microbiological evidence of persistent or recurrent infections showed elevated teicoplanin MICs, among which 14 (8%) concomitantly displayed elevated vancomycin MICs. Clinical, microbiological, and pharmacokinetic variables for patients persistently or recurrently infected with GISA or non-GISA isolates were similar. Bacteremic patients with a poor response to glycopeptide therapy had a 2.8-fold- and 4.8-fold-higher rates of MRSA isolates displaying elevated teicoplanin and vancomycin MICs, respectively, than patients with single isolates (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). Detection of elevated teicoplanin MICs may help to predict a poor response to glycopeptide therapy in MRSA bacteremic patients.\n          <\/jats:p>","DOI":"10.1128\/aac.05808-11","type":"journal-article","created":{"date-parts":[[2011,12,13]],"date-time":"2011-12-13T05:21:18Z","timestamp":1323753678000},"page":"1258-1264","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["High Prevalence of Isolates with Reduced Glycopeptide Susceptibility in Persistent or Recurrent Bloodstream Infections Due to Methicillin-Resistant Staphylococcus aureus"],"prefix":"10.1128","volume":"56","author":[{"given":"Ilker","family":"U\u00e7kay","sequence":"first","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"},{"name":"Infection Control Program, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis","family":"Bernard","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"},{"name":"Infectious Diseases Unit, Bretonneau University Hospital, CHRU of Tours, Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Buzzi","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Harbarth","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"},{"name":"Infection Control Program, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Fran\u00e7ois","sequence":"additional","affiliation":[{"name":"Genomic Research Laboratory, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elzbieta","family":"Huggler","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tristan","family":"Ferry","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"},{"name":"Service des Maladies Infectieuses et Tropicales, H\u00f4pital de la Croix-Rousse, Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Schrenzel","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"},{"name":"Genomic Research Laboratory, Geneva University Hospital and Medical School, Geneva, Switzerland"},{"name":"Central Laboratory of Bacteriology, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Renzoni","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Vaudaux","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel P.","family":"Lew","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Geneva University Hospital and Medical School, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/606027"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.01.012"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000091184.93122.09"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9681(87)90171-8"},{"key":"e_1_3_3_6_2","unstructured":"Clinical Laboratory Standards Institute. 2009. 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