{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:25:51Z","timestamp":1784805951791,"version":"3.55.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n A total of 149 porcine\n Staphylococcus<\/jats:named-content>\n isolates with florfenicol MICs of \u226516 \u03bcg\/ml were screened for the presence of the multiresistance gene\n cfr<\/jats:italic>\n , its location on plasmids, and its genetic environment. In total, 125 isolates carried either\n cfr<\/jats:italic>\n (16 isolates),\n fexA<\/jats:italic>\n (92 isolates), or both genes (17 isolates). The 33\n cfr<\/jats:italic>\n -carrying staphylococci, which included isolates of the species\n Staphylococcus cohnii<\/jats:named-content>\n ,\n S. arlettae<\/jats:named-content>\n , and\n S. saprophyticus<\/jats:named-content>\n in which the\n cfr<\/jats:italic>\n gene has not been described before, exhibited a wide variety of SmaI pulsed-field gel electrophoresis patterns. In 18 cases, the\n cfr<\/jats:italic>\n gene was located on plasmids. Four different types of\n cfr<\/jats:italic>\n -carrying plasmids\u2014pSS-01 (\n n<\/jats:italic>\n = 2; 40 kb), pSS-02 (\n n<\/jats:italic>\n = 3; 35.4 kb), pSS-03 (\n n<\/jats:italic>\n = 10; 7.1 kb), and pBS-01 (\n n<\/jats:italic>\n = 3; 16.4 kb)\u2014were differentiated on the basis of their sizes, restriction patterns, and additional resistance genes. Sequence analysis revealed that in plasmid pSS-01, the\n cfr<\/jats:italic>\n gene was flanked in the upstream part by a complete\n aacA-aphD<\/jats:italic>\n -carrying Tn\n 4001<\/jats:italic>\n -like transposon and in the downstream part by a complete\n fexA<\/jats:italic>\n -carrying transposon Tn\n 558<\/jats:italic>\n . In plasmid pSS-02, an insertion sequence IS\n 21-558<\/jats:italic>\n and the\n cfr<\/jats:italic>\n gene were integrated into transposon Tn\n 558<\/jats:italic>\n and thereby truncated the\n tnpA<\/jats:italic>\n and\n tnpB<\/jats:italic>\n genes. The smallest\n cfr<\/jats:italic>\n -carrying plasmid pSS-03 carried the macrolide-lincosamide-streptogramin B resistance gene\n erm<\/jats:italic>\n (C). Plasmid pBS-01, previously described in\n Bacillus<\/jats:named-content>\n spp., harbored a Tn\n 917<\/jats:italic>\n -like transposon, including the macrolide-lincosamide-streptogramin B resistance gene\n erm<\/jats:italic>\n (B) in the\n cfr<\/jats:italic>\n downstream region. Plasmids, which in part carry additional resistance genes, seem to play an important role in the dissemination of the gene\n cfr<\/jats:italic>\n among porcine staphylococci.\n <\/jats:p>","DOI":"10.1128\/aac.05827-11","type":"journal-article","created":{"date-parts":[[2011,12,20]],"date-time":"2011-12-20T04:50:54Z","timestamp":1324356654000},"page":"1485-1490","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Distribution of the Multidrug Resistance Gene\n cfr<\/i>\n in Staphylococcus Species Isolates from Swine Farms in China"],"prefix":"10.1128","volume":"56","author":[{"given":"Yang","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Development and Evaluation of Chemical and Herbal Drugs for Animal Use, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanjiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Development and Evaluation of Chemical and Herbal Drugs for Animal Use, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Wang","sequence":"additional","affiliation":[{"name":"China Institute of Veterinary Drugs Control, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congming","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Development and Evaluation of Chemical and Herbal Drugs for Animal Use, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangqi","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Development and Evaluation of Chemical and Herbal Drugs for Animal Use, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Development and Evaluation of Chemical and Herbal Drugs for Animal Use, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt-Mariensee, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Development and Evaluation of Chemical and Herbal Drugs for Animal Use, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02260-10"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/656281"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00001-10"},{"key":"e_1_3_2_5_2","unstructured":"Clinical Laboratory Standards Institute. 2008. 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