{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:14:09Z","timestamp":1790396049766,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Elizabethkingia meningoseptica<\/jats:named-content>\n                    , a Gram-negative rod widely distributed in the environment, is resistant to most \u03b2-lactam antibiotics. Three\n                    <jats:italic>bla<\/jats:italic>\n                    genes have been identified in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. meningoseptica<\/jats:named-content>\n                    , coding for the extended-spectrum serine-\u03b2-lactamase CME (class D) and two unrelated wide-spectrum metallo-\u03b2-lactamases, BlaB (subclass B1) and GOB (subclass B3).\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. meningoseptica<\/jats:named-content>\n                    is singular in being the only reported microorganism possessing two chromosomally encoded MBL genes. Real-time PCR and biochemical analysis demonstrate that the three\n                    <jats:italic>bla<\/jats:italic>\n                    genes are actively expressed\n                    <jats:italic>in vivo<\/jats:italic>\n                    as functional \u03b2-lactamases. However, while CME elicits cephalosporin resistance, BlaB is the only \u03b2-lactamase responsible for\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. meningoseptica<\/jats:named-content>\n                    resistance to imipenem, as GOB activity is masked by higher cellular levels of BlaB. On the other hand, we demonstrate that\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>BlaB<\/jats:sub>\n                    expression is higher in the stationary phase or under conditions that mimic the nutrient-limiting cerebrospinal fluid colonized by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. meningoseptica<\/jats:named-content>\n                    in human meningitis.\n                  <\/jats:p>","DOI":"10.1128\/aac.05835-11","type":"journal-article","created":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T02:59:10Z","timestamp":1327978750000},"page":"1686-1692","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Carbapenem Resistance in Elizabethkingia meningoseptica Is Mediated by Metallo-\u03b2-Lactamase BlaB"],"prefix":"10.1128","volume":"56","author":[{"given":"Lisandro J.","family":"Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Instituto de Biologia Molecular y Celular de Rosario, CONICET, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro J.","family":"Vila","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular y Celular de Rosario, CONICET, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.6.2006-2013.1997"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2008.86"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.021600-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2007.05.021"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1878-1886.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.10.3223-3227.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2791-2796.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.1.1-9.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30747-8_25"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1097\/00005792-199701000-00003","article-title":"Chryseobacterium meningosepticum: an emerging pathogen among immunocompromised adults. 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