{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T03:27:20Z","timestamp":1768447640146,"version":"3.49.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility of 17 strains of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mucor circinelloides<\/jats:named-content>\n            to amphotericin B and posaconazole was ascertained by using broth microdilution and disk diffusion methods and by determining the minimal fungicidal concentration (MFC). We evaluated the efficacy of posaconazole at 40 mg\/kg of body weight\/day and amphotericin B at 0.8 mg\/kg\/day in a neutropenic murine model of disseminated infection by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. circinelloides<\/jats:named-content>\n            by using 6 different strains tested previously\n            <jats:italic>in vitro<\/jats:italic>\n            . In general, most of the posaconazole MICs were within the range of susceptibility or intermediate susceptibility, while the small inhibition zone diameters (IZDs) were indicative of nonsusceptibility for all isolates tested. The MFCs were \u22653 dilutions higher than the corresponding MICs. In contrast, amphotericin B showed good activity against all of the strains tested regardless of the method used. The\n            <jats:italic>in vivo<\/jats:italic>\n            studies demonstrated that amphotericin B was effective in prolonging survival and reducing the fungal load. Posaconazole showed poor\n            <jats:italic>in vivo<\/jats:italic>\n            efficacy with no correlation with the MIC values. The results suggested that posaconazole should be used with caution in the treatment of infections caused by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mucor circinelloides<\/jats:named-content>\n            or by strains of\n            <jats:italic>Mucor<\/jats:italic>\n            not identified to the species level.\n          <\/jats:p>","DOI":"10.1128\/aac.05956-11","type":"journal-article","created":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T07:59:10Z","timestamp":1327996750000},"page":"2246-2250","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["<i>In Vitro<\/i>\n            and\n            <i>In Vivo<\/i>\n            Activities of Posaconazole and Amphotericin B in a Murine Invasive Infection by Mucor circinelloides: Poor Efficacy of Posaconazole"],"prefix":"10.1128","volume":"56","author":[{"given":"Valentina","family":"Salas","sequence":"first","affiliation":[{"name":"Unitat de Microbiologia, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Javier","family":"Pastor","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Calvo","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deanna A.","family":"Sutton","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory University of Texas Health Science Center, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"Mayayo","sequence":"additional","affiliation":[{"name":"Unitat de Anatomia Patol\u00f2gica, Facultat de Medicina i Ci\u00e8ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anette W.","family":"Fothergill","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory University of Texas Health Science Center, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Rinaldi","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory University of Texas Health Science Center, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josep","family":"Guarro","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01467-08"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00452-07"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00036-09"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3109\/13693786.2010.499521","article-title":"Two new species of Mucor from clinical samples","volume":"49","author":"Alvarez E","year":"2011","unstructured":"AlvarezE. 2011. Two new species of Mucor from clinical samples. Med. Mycol. 49:62\u201372.","journal-title":"Med. Mycol."},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1080\/13693780801975576","article-title":"Comparative in vitro activities of posaconazole, voriconazole, itraconazole, and amphotericin B against Aspergillus and Rhizopus, and synergy testing for Rhizopus","volume":"46","author":"Arikan S","year":"2008","unstructured":"ArikanS SancakB AlpS HascelikG McNicholasP. 2008. Comparative in vitro activities of posaconazole, voriconazole, itraconazole, and amphotericin B against Aspergillus and Rhizopus, and synergy testing for Rhizopus. Med. Mycol. 46:567\u2013573.","journal-title":"Med. Mycol."},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01685-08"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-006-0107-1"},{"key":"e_1_3_1_9_2","volume-title":"Approved standard","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi. Approved standard, 2nd ed. Document M38-A2. CLSI, Wayne, PA.","edition":"2"},{"key":"e_1_3_1_10_2","volume-title":"Approved guideline","author":"Clinical and Laboratory Standards Institute","year":"2010","unstructured":"Clinical and Laboratory Standards Institute. 2010. Method for antifungal disk diffusion susceptibility testing of nondermatophyte filamentous fungi. Approved guideline. Document M51-A. CLSI, Wayne, PA."},{"key":"e_1_3_1_11_2","volume-title":"Document M51-S1 (proposed)","author":"Clinical and Laboratory Standards Institute","year":"2009","unstructured":"Clinical and Laboratory Standards Institute. 2009. Performance standards for antifungal disk diffusion susceptibility testing of filamentous fungi; informational supplement. Document M51-S1 (proposed). CLSI, Wayne, PA."},{"key":"e_1_3_1_12_2","first-page":"159","volume-title":"New insights in fungal pathogenicity","author":"Dannaoui E","year":"2007","unstructured":"DannaouiE Garc\u00eda-HermosoD. 2007. The zygomycetes, p 159\u2013183. In KavanaghK (ed), New insights in fungal pathogenicity. Springer Science, Dordrecht, the Netherlands."},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.11.3647-3650.2003"},{"key":"e_1_3_1_14_2","first-page":"121","article-title":"In vitro antifungal activities of anidulafungin and micafungin, licensed agents and the investigational triazole posaconazole as determined by NCCLS methods for 12.052 fungal isolate: review of the literature","volume":"20","author":"Espinel-Ingroff A","year":"2003","unstructured":"Espinel-IngroffA. 2003. In vitro antifungal activities of anidulafungin and micafungin, licensed agents and the investigational triazole posaconazole as determined by NCCLS methods for 12.052 fungal isolate: review of the literature. Rev. Iberoam. Micol. 20:121\u2013136.","journal-title":"Rev. Iberoam. Micol."},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01187-06"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3776-3781.2002"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1054\/drup.2000.0171"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr143"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02030-08"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/497078"},{"key":"e_1_3_1_21_2","first-page":"1322","article-title":"Evaluation of disk diffusion and Etest compared to broth microdilution for antifungal susceptibility testing of posaconazole against clinical isolates of filamentous fungi","volume":"45","author":"Messer SA","year":"2007","unstructured":"MesserSA. 2007. Evaluation of disk diffusion and Etest compared to broth microdilution for antifungal susceptibility testing of posaconazole against clinical isolates of filamentous fungi. J. Antimicrob. Microbiol. 45:1322\u20131324.","journal-title":"J. Antimicrob. Microbiol."},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.07.011"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00786-10"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.236"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01463-09"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01026-09"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.3.556-569.2005"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq276"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1581-1582.2002"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2310-2312.2002"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.05956-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.05956-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:54:21Z","timestamp":1645480461000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.05956-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1128\/AAC.05956-11"],"URL":"https:\/\/doi.org\/10.1128\/aac.05956-11","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5]]},"assertion":[{"value":"2011-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}