{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:45:32Z","timestamp":1790966732677,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The presence of SXT\/R391-related integrating conjugative elements (ICEs) in bacterial strains isolated from fish obtained from marine aquaculture environments in 2001 to 2010 in the northwestern Iberian Peninsula was studied. ICEs were detected in 12 strains taxonomically related to\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio scophthalmi<\/jats:named-content>\n                    (3 strains),\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio splendidus<\/jats:named-content>\n                    (5 strains),\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio alginolyticus<\/jats:named-content>\n                    (1 strain),\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Shewanella haliotis<\/jats:named-content>\n                    (1 strain), and\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterovibrio nigricans<\/jats:named-content>\n                    (2 strains), broadening the known host range able to harbor SXT\/R391-like ICEs. Variable DNA regions, which confer element-specific properties to ICEs of this family, were characterized. One of the ICEs encoded antibiotic resistance functions in variable region III, consisting of a tetracycline resistance locus. Interestingly, hot spot 4 included genes providing resistance to rifampin (ICE\n                    <jats:italic>Vsp<\/jats:italic>\n                    Por2 and ICE\n                    <jats:italic>Val<\/jats:italic>\n                    Por1) and quaternary ammonium compounds (QACs) (ICE\n                    <jats:italic>Eni<\/jats:italic>\n                    Spa1), and variable region IV included a mercury resistance operon (ICE\n                    <jats:italic>Vsp<\/jats:italic>\n                    Spa1 and ICE\n                    <jats:italic>Eni<\/jats:italic>\n                    Spa1). The S exclusion group was more represented than the R exclusion group, accounting for two-thirds of the total ICEs. Mating experiments allowed ICE mobilization to\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n                    strains, showing the corresponding transconjugants' rifampin, mercury, and QAC resistance. These results show the first evidence of ICEs providing rifampin and QAC resistances, suggesting that these mobile genetic elements contribute to the dissemination of antimicrobial, heavy metal, and QAC resistance determinants in aquaculture environments.\n                  <\/jats:p>","DOI":"10.1128\/aac.05997-11","type":"journal-article","created":{"date-parts":[[2012,2,7]],"date-time":"2012-02-07T00:51:54Z","timestamp":1328575914000},"page":"2619-2626","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Integrating Conjugative Elements as Vectors of Antibiotic, Mercury, and Quaternary Ammonium Compound Resistance in Marine Aquaculture Environments"],"prefix":"10.1128","volume":"56","author":[{"given":"Arturo","family":"Rodr\u00edguez-Blanco","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Galicia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel L.","family":"Lemos","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Galicia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos R.","family":"Osorio","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Galicia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.11.012"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00518.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2003.10.003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.18.5158-5169.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02094.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90508-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.4.1522-1528.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2006.01.001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2004.01.012"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.11.005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1099\/00221287-76-2-355","article-title":"Transduction of R factors in Proteus mirabilis and P. rettgeri","volume":"76","author":"Coetzee JN","year":"1972","unstructured":"CoetzeeJN DattaN HedgesRW AppelbaumPC. 1972. 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