{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T15:29:44Z","timestamp":1771169384245,"version":"3.50.1"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The increase in drug resistance and invasion caused by biofilm formation brings enormous challenges to the management of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Candida<\/jats:named-content>\n            infection. Aspirin's antibiofilm activity\n            <jats:italic>in vitro<\/jats:italic>\n            was discovered recently. The spectrophotometric method and the XTT {2,3-\n            <jats:italic>bis<\/jats:italic>\n            (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2\n            <jats:italic>H<\/jats:italic>\n            -tetrazolium hydroxide} reduction assay used for data generation make it possible to evaluate fungal biofilm growth accurately. The combined use of the most commonly used methods, the fractional inhibitory concentration index (FICI) and a newly developed method, the \u0394\n            <jats:italic>E<\/jats:italic>\n            model, which uses the concentration-effect relationship over the whole concentration range instead of using the MIC index alone, makes the interpretation of results more reliable. As an attractive tool for studying the pharmacodynamics of antimicrobial agents, time-kill curves can provide detailed information about antimicrobial efficacy as a function of both time and concentration. In the present study,\n            <jats:italic>in vitro<\/jats:italic>\n            interactions between aspirin (acetylsalicylic acid [ASA]) and amphotericin B (AMB) against planktonic cells and biofilm cells of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Candida albicans<\/jats:named-content>\n            and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. parapsilosis<\/jats:named-content>\n            were evaluated by the checkerboard microdilution method and the time-kill test. Synergistic and indifferent effects were found for the combination of ASA and AMB against planktonic cells, while strong synergy was found against biofilm cells analyzed by FICI. The \u0394\n            <jats:italic>E<\/jats:italic>\n            model gave more consistent results with FICI. The positive interactions in concentration were also confirmed by the time-kill test. Moreover, this approach also revealed the pharmacodynamics changes of ASA and synergistic action on time. Our findings suggest a potential clinical use for combination therapy with ASA and AMB to augment activity against biofilm-associated infections.\n          <\/jats:p>","DOI":"10.1128\/aac.06082-11","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T05:49:51Z","timestamp":1331012991000},"page":"3250-3260","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["<i>In Vitro<\/i>\n            Interactions between Aspirin and Amphotericin B against Planktonic Cells and Biofilm Cells of Candida albicans and C. parapsilosis"],"prefix":"10.1128","volume":"56","author":[{"given":"Yabin","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganggang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenshuai","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shikun","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihui","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Key Laboratory for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1335-1343.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1111\/j.1365-2672.2009.04205.x","article-title":"the assessment of the antibacterial and antifungal activities of aspirin, EDTA, and an aspirin-EDTA combination and their effectiveness as antibiofilm agents","volume":"107","author":"Al-Bakri AG","year":"2009","unstructured":"Al-BakriAG OthmanG BustanjiY. 2009. the assessment of the antibacterial and antifungal activities of aspirin, EDTA, and an aspirin-EDTA combination and their effectiveness as antibiofilm agents. J. Appl. Microbiol. 107:280\u2013286.","journal-title":"J. Appl. Microbiol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.41-47.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46172-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46569-0"},{"key":"e_1_3_2_7_2","first-page":"197","article-title":"In search of the holy grail of antifungal therapy","volume":"119","author":"Chapman SW","year":"2008","unstructured":"ChapmanSW SullivanDC ClearyJD. 2008. In search of the holy grail of antifungal therapy. Trans. Am. Clin. Climatol. Assoc. 119:197\u2013216.","journal-title":"Trans. Am. Clin. Climatol. Assoc."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00041-08"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.506-508.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.2174\/138945006776359458"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-64402008000400014"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10616.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.2.167-193.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(98)00099-6"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3846-3853.2002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2475"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.2.536-545.2004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0065-2911(02)46005-5","article-title":"The physiology and collective recalcitrance of microbial biofilm communities","volume":"46","author":"Gilbert P","year":"2002","unstructured":"GilbertP Maira-LitranT McBainAJ RickardAH WhyteFW. 2002. The physiology and collective recalcitrance of microbial biofilm communities. Adv. Microb. Physiol. 46:203\u2013256.","journal-title":"Adv. Microb. Physiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1128\/AAC.01638-09","article-title":"Interaction of Candida albicans biofilms with antifungals: transcriptional response and binding of antifungals to \u03b2-glucans","volume":"54","author":"Govindsamy V","year":"2010","unstructured":"GovindsamyV RossignolT d'EnfertC. 2010. Interaction of Candida albicans biofilms with antifungals: transcriptional response and binding of antifungals to \u03b2-glucans. Antimicrob. Agents Chemother. 54:2096\u20132111.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27663-0"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.04.011"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1196\/annals.1406.019","article-title":"Amphotericin B and coccidioidomicosis","volume":"1111","author":"Johnson RH","year":"2007","unstructured":"JohnsonRH EinsteinHE. 2007. Amphotericin B and coccidioidomicosis. Ann. N. Y. Acad. Sci. 1111:434\u2013441.","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2009.06.003"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00902.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.1.106-117.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4256-4263.2001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.1.163-194.2005"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4333-4340.2003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01056-06"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020063"},{"key":"e_1_3_2_31_2","unstructured":"National Committee for Clinical Laboratory Standards. 2002. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard. Document M27-A2. NCCLS Wayne PA."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg301"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1099\/0022-1317-49-9-831","article-title":"Antifungal activity of ibuprofen alone and in combination with fluconazole against Candida species","volume":"49","author":"Pina-Vaz C","year":"2000","unstructured":"Pina-VazC. 2000. Antifungal activity of ibuprofen alone and in combination with fluconazole against Candida species. J. Med. Microbiol. 49:831\u2013840.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.1999.00475.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00117.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.9.2475-2479.2001"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002257"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00181-0"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1586\/14787210.3.3.361","article-title":"Pharmacokinetic\/pharmacodynamic evaluation of anti-infective agents","volume":"3","author":"Schuck EL","year":"2005","unstructured":"SchuckEL DerendorfH. 2005. Pharmacokinetic\/pharmacodynamic evaluation of anti-infective agents. Expert Rev. Anti-Infect. Ther. 3:361\u2013373.","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.12.2610"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01070-07"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2004.16.2.134"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05321-1"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2006.08.069"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2982-2989.2002"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00013-08"},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.micinf.2009.08.007","article-title":"Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases","volume":"11","author":"Trofa D","year":"2009","unstructured":"TrofaD. 2009. Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases. Microbes Infect. 11:1131\u20131139.","journal-title":"Microbes Infect."},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1136\/gut.52.4.490","article-title":"Aspirin inhibits the growth of Helicobacter pylori and enhances its susceptibility to antimicrobial agents","volume":"52","author":"Wang W","year":"2003","unstructured":"WangW. 2003. Aspirin inhibits the growth of Helicobacter pylori and enhances its susceptibility to antimicrobial agents. Gut 52:490\u2013495.","journal-title":"Gut"},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1161\/01.CIR.102.17.2022","article-title":"Aspirin and salicylate: an old remedy with a new twist","volume":"102","author":"Wu KK","year":"2000","unstructured":"WuKK. 2000. Aspirin and salicylate: an old remedy with a new twist. Circulation 102:2022\u20132023.","journal-title":"Circulation"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28959-0"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.06082-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.06082-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:11:49Z","timestamp":1645481509000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.06082-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1128\/AAC.06082-11"],"URL":"https:\/\/doi.org\/10.1128\/aac.06082-11","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6]]},"assertion":[{"value":"2011-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-05-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}