{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:11:36Z","timestamp":1791292296309,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The spread of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            gene is gaining worldwide attentions. This gene is usually carried by large plasmids and has been discovered in diverse bacteria since it was originally found in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            . Here we report the complete sequences of a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            -bearing plasmid, pNDM-BJ01, and its variant, pNDM-BJ02, isolated from clinical\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter lwoffii<\/jats:named-content>\n            strains. The plasmid pNDM-BJ01 is 47.3 kb in size and cannot be classified into any known plasmid incompatibility group, thus representing a novel plasmid with an unknown maintenance mechanism. This plasmid contains both a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            gene and a type IV secretion system (T4SS) gene cluster. The T4SS is assigned to the P-type T4SS group, which usually encode a short, rigid pilus, and the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            gene is located within a composite transposon flanked by two insertion elements of IS\n            <jats:italic>Aba125<\/jats:italic>\n            . Plasmid pNDM-BJ02 is nearly identical to pNDM-BJ01 except that one copy of the IS\n            <jats:italic>Aba125<\/jats:italic>\n            element is missing, and it is therefore regarded as a variant of pNDM-BJ01. Sequence alignment indicated that this\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            -containing composite transposon, which can also be captured by other mobile elements, was probably a product of multiple recombination events and can move as a whole by transposition.\n          <\/jats:p>","DOI":"10.1128\/aac.06199-11","type":"journal-article","created":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T07:59:10Z","timestamp":1327996750000},"page":"1698-1702","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":132,"title":["Novel Plasmid and Its Variant Harboring both a\n            <i>bla<\/i>\n            <sub>NDM-1<\/sub>\n            Gene and Type IV Secretion System in Clinical Isolates of Acinetobacter lwoffii"],"prefix":"10.1128","volume":"56","author":[{"given":"Hongyan","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, The General Hospital of Chinese People's Armed Police Forces, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfei","family":"Hu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanlong","family":"Pan","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The General Hospital of Chinese People's Armed Police Forces, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The General Hospital of Chinese People's Armed Police Forces, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The General Hospital of Chinese People's Armed Police Forces, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinfang","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The General Hospital of Chinese People's Armed Police Forces, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The General Hospital of Chinese People's Armed Police Forces, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Xiao","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunguang","family":"Luan","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Peking Union 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Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajun","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoli","family":"Zhu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq179"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_4_2","first-page":"750","article-title":"Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase\u2014United States","volume":"59","author":"Centers for Disease 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