{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:45:44Z","timestamp":1791398744088,"version":"4.3.3"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We identified a clinical isolate of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Candida glabrata<\/jats:named-content>\n            (CG156) exhibiting flocculent growth and cross-resistance to fluconazole (FLC), voriconazole (VRC), and amphotericin B (AMB), with MICs of &gt;256, &gt;256, and 32 \u03bcg ml\n            <jats:sup>\u22121<\/jats:sup>\n            , respectively. Sterol analysis using gas chromatography-mass spectrometry (GC-MS) revealed that CG156 was a sterol 14\u03b1-demethylase (Erg11p) mutant, wherein 14\u03b1-methylated intermediates (lanosterol was &gt;80% of the total) were the only detectable sterols.\n            <jats:italic>ERG11<\/jats:italic>\n            sequencing indicated that CG156 harbored a single-amino-acid substitution (G315D) which nullified the function of native Erg11p. In heterologous expression studies using a doxycycline-regulatable\n            <jats:italic>Saccharomyces cerevisiae erg11<\/jats:italic>\n            strain, wild-type\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. glabrata<\/jats:named-content>\n            Erg11p fully complemented the function of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. cerevisiae<\/jats:named-content>\n            sterol 14\u03b1-demethylase, restoring growth and ergosterol synthesis in recombinant yeast; mutated CG156 Erg11p did not. CG156 was culturable using sterol-free, glucose-containing yeast minimal medium (\n            <jats:sub>glc<\/jats:sub>\n            YM). However, when grown on sterol-supplemented\n            <jats:sub>glc<\/jats:sub>\n            YM (with ergosta 7,22-dienol, ergosterol, cholestanol, cholesterol, \u0394\n            <jats:sup>7<\/jats:sup>\n            -cholestenol, or desmosterol), CG156 cultures exhibited shorter lag phases, reached higher cell densities, and showed alterations in cellular sterol composition. Unlike comparator isolates (harboring wild-type\n            <jats:italic>ERG11<\/jats:italic>\n            ) that became less sensitive to FLC and VRC when cultured on sterol-supplemented\n            <jats:sub>glc<\/jats:sub>\n            YM, facultative sterol uptake by CG156 did not affect its azole-resistant phenotype. Conversely, CG156 grown using\n            <jats:sub>glc<\/jats:sub>\n            YM with ergosterol (or with ergosta 7,22-dienol) showed increased sensitivity to AMB; CG156 grown using\n            <jats:sub>glc<\/jats:sub>\n            YM with cholesterol (or with cholestanol) became more resistant (MICs of 2 and &gt;64 \u03bcg AMB ml\n            <jats:sup>\u22121<\/jats:sup>\n            , respectively). Our results provide insights into the consequences of sterol uptake and metabolism on growth and antifungal resistance in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. glabrata<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.06253-11","type":"journal-article","created":{"date-parts":[[2012,5,22]],"date-time":"2012-05-22T02:55:06Z","timestamp":1337655306000},"page":"4223-4232","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":112,"title":["Facultative Sterol Uptake in an Ergosterol-Deficient Clinical Isolate of Candida glabrata Harboring a Missense Mutation in\n            <i>ERG11<\/i>\n            and Exhibiting Cross-Resistance to Azoles and Amphotericin B"],"prefix":"10.1128","volume":"56","author":[{"given":"Claire M.","family":"Hull","sequence":"first","affiliation":[{"name":"Institute of Life Science and College of Medicine, Swansea University, Swansea, Wales, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josie E.","family":"Parker","sequence":"additional","affiliation":[{"name":"Institute of Life Science and College of Medicine, Swansea University, Swansea, Wales, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Bader","sequence":"additional","affiliation":[{"name":"University Medical Center G\u00f6ttingen, Institute for Medical Microbiology and German National Reference Center for Systemic Mycoses, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Weig","sequence":"additional","affiliation":[{"name":"University Medical Center G\u00f6ttingen, Institute for Medical Microbiology and German National Reference Center for Systemic Mycoses, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Gross","sequence":"additional","affiliation":[{"name":"University Medical Center G\u00f6ttingen, Institute for Medical Microbiology and German National Reference Center for Systemic Mycoses, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew G. 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