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We sequenced the biosynthetic gene cluster responsible for fosfomycin production in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas syringae<\/jats:named-content>\n                    PB-5123. Surprisingly, the biosynthetic pathway in this organism is very different from that in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Streptomyces fradiae<\/jats:named-content>\n                    and\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Streptomyces wedmorensis<\/jats:named-content>\n                    . The pathways share the first and last steps, involving conversion of phosphoenolpyruvate to phosphonopyruvate (PnPy) and 2-hydroxypropylphosphonate (2-HPP) to fosfomycin, respectively, but the enzymes converting PnPy to 2-HPP are different. The genome of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. syringae<\/jats:named-content>\n                    PB-5123 lacks a gene encoding the PnPy decarboxylase found in the\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Streptomyces<\/jats:named-content>\n                    strains. Instead, it contains a gene coding for a citrate synthase-like enzyme, Psf2, homologous to the proteins that add an acetyl group to PnPy in the biosynthesis of FR-900098 and phosphinothricin. Heterologous expression and purification of Psf2 followed by activity assays confirmed the proposed activity of Psf2. Furthermore, heterologous production of fosfomycin in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>\n                    from a fosmid encoding the fosfomycin biosynthetic cluster from\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. syringae<\/jats:named-content>\n                    PB-5123 confirmed that the gene cluster is functional. Therefore, two different pathways have evolved to produce this highly potent antimicrobial agent.\n                  <\/jats:p>","DOI":"10.1128\/aac.06478-11","type":"journal-article","created":{"date-parts":[[2012,5,21]],"date-time":"2012-05-21T22:55:06Z","timestamp":1337640906000},"page":"4175-4183","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Different Biosynthetic Pathways to Fosfomycin in Pseudomonas syringae and Streptomyces Species"],"prefix":"10.1128","volume":"56","author":[{"given":"Seung Young","family":"Kim","sequence":"first","affiliation":[{"name":"Institute of Genomic Biology, University of Illinois at Urbana\u2014Champaign, Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kou-San","family":"Ju","sequence":"additional","affiliation":[{"name":"Institute of Genomic Biology, University of Illinois at Urbana\u2014Champaign, Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William W.","family":"Metcalf","sequence":"additional","affiliation":[{"name":"Institute of Genomic Biology, University of Illinois at Urbana\u2014Champaign, Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley S.","family":"Evans","sequence":"additional","affiliation":[{"name":"Institute of Genomic Biology, University of Illinois at Urbana\u2014Champaign, Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohisa","family":"Kuzuyama","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, University of Tokyo, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfred A.","family":"van der Donk","sequence":"additional","affiliation":[{"name":"Institute of Genomic Biology, University of Illinois at Urbana\u2014Champaign, Urbana, Illinois, USA"},{"name":"Howard Hughes Medical Institute and Department of Chemistry, University of Illinois at Urbana\u2014Champaign, Urbana, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1159\/000238812","article-title":"Brief overview of single-dose therapy for uncomplicated urinary tract infections","volume":"36","author":"Bailey RR","year":"1990","unstructured":"BaileyRR. 1990. 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