{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T15:50:46Z","timestamp":1722009046653},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1976,7,1]],"date-time":"1976-07-01T00:00:00Z","timestamp":205027200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1976,7]]},"abstract":"<jats:p>\n            The minimal inhibitory concentrations of gentamicin and minocycline alone and in combination were determined by a broth microdilution method for 100 aerobic, facultative, and anaerobic isolates representative of pathogens recovered from patients with intra-abdominal sepsis. Gentamicin inhibited all strains of\n            <jats:italic>Klebsiella, Enterobacter<\/jats:italic>\n            , and\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            in concentrations of 0.4 to 3.1 \u03bcg\/ml and all strains of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            in concentrations of 0.8 to 12.5 \u03bcg\/ml. Whereas minocycline did not consistently inhibit these organisms in concentrations of 1.6 \u03bcg or less\/ml, it did act synergistically with gentamicin against 43% of the\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            tested in clinically achievable concentrations; significant synergy was most common with\n            <jats:italic>E. coli<\/jats:italic>\n            (60%). Minocycline inhibited 62% of\n            <jats:italic>Bacteroides fragilis<\/jats:italic>\n            , 71% of\n            <jats:italic>Clostridium<\/jats:italic>\n            , 40% of anaerobic cocci, and 40% of enterococci tested in concentrations of 1.6 \u03bcg or less\/ml. Whereas gentamicin rarely inhibited these organisms in concentrations of 6.2 \u03bcg or less\/ml, it did act synergistically with minocycline against 20% of\n            <jats:italic>B. fragilis<\/jats:italic>\n            , 67% of\n            <jats:italic>Clostridium<\/jats:italic>\n            , 22% of anaerobic cocci, and 22% of enterococci (which had minimal inhibitory concentrations of minocycline within the range tested) at clinically achievable concentrations. Although only four (13%) of the 30 isolates resistant to both gentamicin and minocycline alone were inhibited by clinically achievable concentrations of the combination, the observed synergy, particularly against strains of\n            <jats:italic>E. coli<\/jats:italic>\n            , was considered to be of potential clinical usefulness. Antagonism between gentamicin and minocycline was not observed at the concentrations tested.\n          <\/jats:p>","DOI":"10.1128\/aac.10.1.34","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"34-37","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["In Vitro Activity of Gentamicin and Minocycline Alone and in Combination Against Bacteria Associated with Intra-Abdominal Sepsis"],"prefix":"10.1128","volume":"10","author":[{"given":"Robert J.","family":"Fass","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, The Ohio State University College of Medicine, Columbus, Ohio 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel E.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, The Ohio State University College of Medicine, Columbus, Ohio 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard B.","family":"Prior","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, The Ohio State University College of Medicine, Columbus, Ohio 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Perkins","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, The Ohio State University College of Medicine, Columbus, Ohio 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.10.1.34","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.10.1.34","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T13:34:53Z","timestamp":1645450493000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.10.1.34"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1976,7]]}},"alternative-id":["10.1128\/AAC.10.1.34"],"URL":"https:\/\/doi.org\/10.1128\/aac.10.1.34","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,7]]},"assertion":[{"value":"1976-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}