{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:29:29Z","timestamp":1785475769826,"version":"3.56.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1976,8,1]],"date-time":"1976-08-01T00:00:00Z","timestamp":207705600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1976,8]]},"abstract":"<jats:p>\n            An agar dilution method was employed to test the susceptibility of 12 strains of\n            <jats:italic>Coccidioides immitis<\/jats:italic>\n            to polymyxin B (PB). After 3 days of incubation, eight strains were markedly inhibited by 5.0 \u03bcg of PB per ml and four strains did not grow. PB at 10 \u03bcg\/ml inhibited the growth of all strains through 20 days of incubation. To determine whether PB has anticoccidioidal activity in vivo, mice in groups of 30 were infected intraperitoneally with a mean lethal dose (LD\n            <jats:sub>50<\/jats:sub>\n            ) or 20 LD\n            <jats:sub>50<\/jats:sub>\n            of arthrospores of\n            <jats:italic>C. immitis<\/jats:italic>\n            ATCC 28868 (Silveira). Treatment by intraperitoneal injection of PB (2.5 mg\/kg) was begun 2 or 5 days after challenge. By 40 days after infection, 47 and 7% of the untreated mice challenged with an LD\n            <jats:sub>50<\/jats:sub>\n            , respectively, were alive. Of mice infected with an LD\n            <jats:sub>50<\/jats:sub>\n            or 20 LD\n            <jats:sub>50<\/jats:sub>\n            and treated with PB beginning 2 days after the challenge, 90% of each group were alive by day 40. Initiation of PB therapy 5 days after infection permitted survival of 84% of the mice infected with an LD\n            <jats:sub>50<\/jats:sub>\n            ; however, only 27% of the mice infected with 20 LD\n            <jats:sub>50<\/jats:sub>\n            survived by day 40. In this latter group there was evidence that PB prolonged life since 56% of the treated mice were alive by day 15 as compared with 30% of the controls. PB in vivo was fungistatic since the majority of treated mice had\n            <jats:italic>C. immitis<\/jats:italic>\n            in the liver, lungs, and spleen.\n          <\/jats:p>","DOI":"10.1128\/aac.10.2.318","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"318-321","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Treatment of Murine Coccidioidomycosis with Polymyxin B"],"prefix":"10.1128","volume":"10","author":[{"given":"M. S.","family":"Collins","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine, University of California, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Pappagianis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine, University of California, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.10.2.318","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.10.2.318","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:14:09Z","timestamp":1645485249000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.10.2.318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1976,8]]}},"alternative-id":["10.1128\/AAC.10.2.318"],"URL":"https:\/\/doi.org\/10.1128\/aac.10.2.318","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,8]]},"assertion":[{"value":"1976-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}