{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T13:57:00Z","timestamp":1783778220369,"version":"3.55.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1977,8,1]],"date-time":"1977-08-01T00:00:00Z","timestamp":239241600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1977,8]]},"abstract":"<jats:p>\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            strain GC82 contains a plasmid specifying a \u03b2-lactamase (\u03b2-Lam\n            <jats:sup>+<\/jats:sup>\n            ). Mixed incubation of strain GC82 with a penicillin-susceptible (\u03b2-Lam\n            <jats:sup>\u2212<\/jats:sup>\n            ), streptomycin-resistant mutant of strain GC9 results in the expression of \u03b2-lactamase activity and streptomycin resistance in the transcipients. The frequency of transfer of the plasmid-specified resistance to penicillin seems to be proportional to the initial input ratio of the mating mixture of donor to recipient and to correlate positively with bacterial density. Cell-to-cell transmission of the deoxyribonucleic acid (DNA) appears to be by a conjugal mechanism or, alternatively, by an as yet undescribed transducing phage. Additionally, whole-cell DNA from a \u03b2-lactamase-producing strain could be used to transform streptomycin-resistant recipients, resulting in the expression of both \u03b2-lactamase activity and streptomycin resistance in the transformants, and purified gonococcal plasmid DNA transformed\n            <jats:italic>Escherichia coli<\/jats:italic>\n            but not the gonococcus. Circular DNA extracted from donor GC82 comprised three molecular species (approximately 2.7, 4.8, and 25 megadaltons [Mdal]), whereas the recipients GC9-S (Str\n            <jats:sup>r<\/jats:sup>\n            ) contained only the 2.7-Mdal cryptic DNA species. DNA from the GC9-S82 (Str\n            <jats:sup>r<\/jats:sup>\n            , \u03b2-Lam\n            <jats:sup>+<\/jats:sup>\n            ) transcipient contained a 4.8-Mdal species in addition to the cryptic molecular species (2.7 Mdal). The finding that the transcipient will not retransfer \u03b2-lactamase is consistent with the hypothesis that the 25-Mdal plasmid promotes mobilization of the smaller 4.8-Mdal R plasmid.\n          <\/jats:p>","DOI":"10.1128\/aac.12.2.270","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"270-280","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Transfer of Plasmid-Borne Beta-Lactamase in\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"12","author":[{"given":"Ellen S.","family":"Baron","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur K.","family":"Saz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Schools of Medicine and Dentistry, Georgetown University, Washington, D.C. 20007"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis J.","family":"Kopecko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Wohlhieter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.12.2.270","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.12.2.270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:14:46Z","timestamp":1645485286000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.12.2.270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1977,8]]}},"alternative-id":["10.1128\/AAC.12.2.270"],"URL":"https:\/\/doi.org\/10.1128\/aac.12.2.270","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,8]]},"assertion":[{"value":"1977-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}