{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:43:14Z","timestamp":1648600994890},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1978,2,1]],"date-time":"1978-02-01T00:00:00Z","timestamp":255139200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1978,2]]},"abstract":"<jats:p>\n            It is generally accepted that strains of\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            which are susceptible to penicillin G do not produce \u03b2-lactamase. However, we have found that such a strain susceptible to 0.06 \u03bcg of penicillin per ml and 0.56 \u03bcg of methicillin per ml produces \u03b2-lactamase(s) which hydrolyzes penicillin G, methicillin, 6-aminopenicillanic acid, and probably cephaloridine. The enzyme which is found only during very early log phase of the growth cycle is not inducible either by penicillin or methicillin and is cell bound and liberated only by disruption of the cell. The rate of enzymatic hydrolysis of methicillin was 60% that of benzylpenicillin. This finding suggests that the elaboration per se of \u03b2-lactamase does not necessarily afford resistance to penicillin in this gram-positive-producing cell.\n          <\/jats:p>","DOI":"10.1128\/aac.13.2.289","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"289-292","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Isolation of Beta-Lactamase from a Penicillin-Susceptible Strain of\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"S.","family":"Sachithanandam","sequence":"first","affiliation":[{"name":"Department of Microbiology, Georgetown University, Schools of Medicine and Dentistry, Washington, D.C. 20007"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. L.","family":"Lowery","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Georgetown University, Schools of Medicine and Dentistry, Washington, D.C. 20007"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. K.","family":"Saz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Georgetown University, Schools of Medicine and Dentistry, Washington, D.C. 20007"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.13.2.289","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.13.2.289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:14:58Z","timestamp":1645485298000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.13.2.289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1978,2]]}},"alternative-id":["10.1128\/AAC.13.2.289"],"URL":"https:\/\/doi.org\/10.1128\/aac.13.2.289","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,2]]},"assertion":[{"value":"1978-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}