{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:09:15Z","timestamp":1790651355943,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1979,2,1]],"date-time":"1979-02-01T00:00:00Z","timestamp":286675200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1979,2]]},"abstract":"<jats:p>\n            A strain of\n            <jats:italic>Trichomonas vaginalis<\/jats:italic>\n            (IR-78), recently isolated from a patient afflicted with recurrent symptomatic trichomoniasis, showed resistance to metronidazole, tinidazole, and nimorazole in vitro as well as in vivo. In a serial dilution test using cysteine monohydrochloride\u2013peptone\u2013liver infusion\u2013maltose medium,\n            <jats:italic>T. vaginalis<\/jats:italic>\n            IR-78 was only resistant under aerobic conditions. Under anaerobic conditions it was as susceptible as the normal reference strain. The minimal lethal concentrations of metronidazole, tinidazole, and nimorazole for IR-78 were 100, 50, and 50 \u03bcg\/ml aerobically and 0.4, 0.4, and 0.2 \u03bcg\/ml ana\u00e9robically, respectively. The efficacy of metronidazole, tinidazole, and nimorazole was assessed in vivo by oral administration to mice simultaneously infected with IR-78 both subcutaneously and intraperitoneally. The CD\n            <jats:sub>50<\/jats:sub>\n            (dose needed to cure 50% of infections) of each compound was significantly higher for the subcutaneous than for the intraperitoneal infection. In contrast, there was little difference in CD\n            <jats:sub>50<\/jats:sub>\n            for these infections in mice inoculated with a susceptible trichomonas strain. The CD\n            <jats:sub>50<\/jats:sub>\n            's for all three compounds against intraperitoneal and subcutaneous infections with IR-78 were 2 to &gt;70 times higher than for susceptible strain E. Both forms of infection with IR-78 could always be cured with therapeutically acceptable doses of tinidazole and nimorazole; subcutaneous infections could not be cured with tolerated doses of metronidazole.\n          <\/jats:p>","DOI":"10.1128\/aac.15.2.254","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"254-257","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":121,"title":["Strain of\n            <i>Trichomonas vaginalis<\/i>\n            Resistant to Metronidazole and Other 5-Nitroimidazoles"],"prefix":"10.1128","volume":"15","author":[{"given":"Josef G.","family":"Meingassner","sequence":"first","affiliation":[{"name":"Sandoz Forschungsinstitut, A-1235 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefine","family":"Thurner","sequence":"additional","affiliation":[{"name":"II. Universit\u00e4ts-Hautklinik, A-1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.15.2.254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.15.2.254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:16:01Z","timestamp":1645485361000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.15.2.254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1979,2]]}},"alternative-id":["10.1128\/AAC.15.2.254"],"URL":"https:\/\/doi.org\/10.1128\/aac.15.2.254","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,2]]},"assertion":[{"value":"1979-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}