{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T09:32:04Z","timestamp":1698831124736},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1979,4,1]],"date-time":"1979-04-01T00:00:00Z","timestamp":291772800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1979,4]]},"abstract":"<jats:p>We recently found that bilirubin interferes with the colorimetric chloramphenicol assay of Kakemi et al. (K. T. Kakemi, T. Arita, and S. Ohashi, Yakugaku Zasshi 82:342\u2013345, 1962). Levels of serum bilirubin alone (4 to 6 mg\/dl) resulted in apparent concentrations of chloramphenicol which appear to be in the therapeutic range. Concentrations of serum bilirubin greater than 8 mg\/dl resulted in levels of apparent chloramphenicol associated with toxicity (&gt;50 \u03bcg\/ml). Small amounts of activated charcoal added to the isoamyl acetate extraction step of the assay eliminated this interference by bilirubin.<\/jats:p>","DOI":"10.1128\/aac.15.4.544","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"544-546","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Modification of the Colorimetric Assay for Chloramphenicol in the Presence of Bilirubin"],"prefix":"10.1128","volume":"15","author":[{"suffix":"Jr.","given":"Edward O.","family":"Mason","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Baylor College of Medicine, and Laura C. Bickel, M.D.-Charles Thomas Parker Laboratory, Texas Children's Hospital, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheldon L.","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Baylor College of Medicine, and Laura C. Bickel, M.D.-Charles Thomas Parker Laboratory, Texas Children's Hospital, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol J.","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Baylor College of Medicine, and Laura C. Bickel, M.D.-Charles Thomas Parker Laboratory, Texas Children's Hospital, Houston, Texas 77030"},{"name":"Department of Microbiology and Immunology, Baylor College of Medicine, and Laura C. Bickel, M.D.-Charles Thomas Parker Laboratory, Texas Children's Hospital, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy A.","family":"Ritter","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Baylor College of Medicine, and Laura C. Bickel, M.D.-Charles Thomas Parker Laboratory, Texas Children's Hospital, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.15.4.544","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.15.4.544","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T13:53:46Z","timestamp":1645451626000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.15.4.544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1979,4]]}},"alternative-id":["10.1128\/AAC.15.4.544"],"URL":"https:\/\/doi.org\/10.1128\/aac.15.4.544","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,4]]},"assertion":[{"value":"1979-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}