{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:49:42Z","timestamp":1759682982343},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1979,4,1]],"date-time":"1979-04-01T00:00:00Z","timestamp":291772800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1979,4]]},"abstract":"<jats:p>\n            The minimal inhibitory concentrations of 30 antibiotics were determined by the agar dilution method for 17 penicillinase-positive and 50 penicillinase-negative strains of\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            . The latter included 42 strains that were penicillin susceptible (pen S) (minimal inhibitory concentration, &lt;0.125 \u03bcg\/ml) and 8 strains with intermediate resistance to penicillin (pen I; minimal inhibitory concentration, 0.125 to 0.5 \u03bcg\/ml). Two penicillinase-resistant penicillins (methicillin and nafcillin) were inhibitory for penicillin-resistant (pen R) strains. Three new cephalosporins (cefuroxime, cefamandole, cefaclor) and a cephamycin (cefoxitin) were bacteriostatic (minimal inhibitory concentration \u22640.8 \u03bcg\/ml) for 90% of pen S, pen I, and pen R strains. Pen I strains were more resistant than pen R strains to 6 of 13 cephalosporins. Rifampin, erythromycin, spectinomycin, chloramphenicol, and the tetracyclines were inhibitory for both pen S and pen R strains. The minimal bactericidal concentrations of cefuroxime, cefamandole, cefaclor, and cefoxitin were measured for 17 pen R strains and eight pen I strains by serial dilution of the antibiotics in Trypticase soy broth supplemented with 1% Iso VitaleX and 1% hemoglobin. All tubes were subcultured after overnight incubation at 37\u00b0C. Cefuroxime and cefoxitin were bactericidal at low concentrations (minimal bactericidal concentration, \u22641.0 \u03bcg\/ml) for 16 of 17 pen R strains and 6 of 8 pen I strains.\n          <\/jats:p>","DOI":"10.1128\/aac.15.4.562","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"562-567","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Comparative Susceptibility of Penicillinase-Positive and -Negative\n            <i>Neisseria gonorrhoeae<\/i>\n            to 30 Antibiotics"],"prefix":"10.1128","volume":"15","author":[{"given":"Wendell H.","family":"Hall","sequence":"first","affiliation":[{"name":"Infectious Disease Section, Department of Medicine, Veterans Administration Medical Center, Minneapolis, Minnesota 554171"},{"name":"University of Minnesota Medical School, Minneapolis, Minnesota 55455"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Schierl","sequence":"additional","affiliation":[{"name":"Infectious Disease Section, Department of Medicine, Veterans Administration Medical Center, Minneapolis, Minnesota 554171"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E.","family":"Maccani","sequence":"additional","affiliation":[{"name":"Infectious Disease Section, Department of Medicine, Veterans Administration Medical Center, Minneapolis, Minnesota 554171"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.15.4.562","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.15.4.562","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T13:53:35Z","timestamp":1645451615000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.15.4.562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1979,4]]}},"alternative-id":["10.1128\/AAC.15.4.562"],"URL":"https:\/\/doi.org\/10.1128\/aac.15.4.562","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,4]]},"assertion":[{"value":"1979-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}