{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T14:21:47Z","timestamp":1702390907892},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1979,7,1]],"date-time":"1979-07-01T00:00:00Z","timestamp":299635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1979,7]]},"abstract":"<jats:p>\n            The synthesis in vitro of peptidoglycan by\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            was studied in organisms made permeable to nucleotide precursors by treatment with ether. Optimum synthesis occurred at 30\u00b0C in tris(hydroxymethyl)aminomethane-maleate buffer (0.05 M; pH 6) in the presence of 20 mM Mg\n            <jats:sup>2+<\/jats:sup>\n            . The incorporation from uridine 5\u2032-diphosphate-\n            <jats:italic>N<\/jats:italic>\n            -acetyl-[\n            <jats:sup>14<\/jats:sup>\n            C]glucosamine into peptidoglycan, measured after precipitation of the cells with trichloroacetic acid, was sensitive to the \u03b2-lactam antibiotics, bacitracin, diumycin, and tunicamycin and relatively resistant to spectinomycin and tetracycline. Differences in sensitivity between preparations from a \u03b2-lactamase producer and a laboratory segregant derived from it were not great. Synthesized peptidoglycan was also fractionated into sodium dodecyl sulfate-soluble and -insoluble portions. \u03b2-Lactam antibiotics at concentrations equivalent to the minimal inhibitory concentrations for growth of the organisms did not inhibit peptidoglycan synthesis, but rather caused a small enhancement. At higher concentrations, above about 0.5 \u03bcg\/ml, incorporation into sodium dodecyl sulfate-insoluble material was progressively inhibited, whereas the amount of sodium dodecyl sulfate-soluble product increased greatly, more than compensating for the loss of the precipitable fraction. Similar observations were made with three strains, and also with the \u03b2-lactam clavulanic acid, normally considered as a \u03b2-lactamase inhibitor rather than as itself an effective antibiotic.\n          <\/jats:p>","DOI":"10.1128\/aac.16.1.28","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"28-36","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["In Vitro Synthesis of Peptidoglycan by \u03b2-Lactam-Sensitive and -Resistant Strains of\n            <i>Neisseria gonorrhoeae<\/i>\n            : Effects of \u03b2-Lactam and Other Antibiotics"],"prefix":"10.1128","volume":"16","author":[{"given":"Christine A.","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Microbiology, Life Sciences Building, University of Liverpool, Liverpool L69 3BX, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold R.","family":"Perkins","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Life Sciences Building, University of Liverpool, Liverpool L69 3BX, England"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.16.1.28","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.16.1.28","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:15:42Z","timestamp":1645485342000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.16.1.28"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1979,7]]}},"alternative-id":["10.1128\/AAC.16.1.28"],"URL":"https:\/\/doi.org\/10.1128\/aac.16.1.28","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,7]]},"assertion":[{"value":"1979-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}